Thrombin preconditioning upregulates transferrin and transferrin receptor and reduces brain edema induced by lysed red blood cells.
Thrombin preconditioning upregulates transferrin and transferrin receptor and reduces brain edema induced by lysed red blood cells.
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DOI：
10.1007/978-3-7091-0651-8_92
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
G. Xi;J. Wu;Y. Jiang;Y. Hua;R. Keep;J. Hoff
中科院分区：
文献类型：
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作者：
G. Xi;J. Wu;Y. Jiang;Y. Hua;R. Keep;J. Hoff
使用低剂量凝血酶进行预处理可减少出血性和缺血性中风后的脑水肿。我们将这种现象称为凝血酶预处理（TPC）或凝血酶诱导的脑耐受。本研究探讨 TPC 是否可以减轻裂解红细胞 (RBC) 引起的脑水肿，以确定脑出血 (ICH) 早期凝血酶的产生是否可能改变与血凝块溶解相关的潜在伤害事件。它还检查 TPC 是否可能通过改变大脑内的铁处理，特别是通过调节转铁蛋白 (Tf) 和转铁蛋白受体 (TfR) 水平来发挥保护作用。通过湿/干重测量脑水肿。使用蛋白质印迹分析和免疫组织化学进行 Tf 和 TfR 测量。我们发现 TPC 可以减少裂解红细胞引起的脑水肿，并上调大脑中 Tf 和 TfR 的水平。 ICH后凝血酶的形成可能是信号级联的一部分，该信号级联通过改变铁处理蛋白来限制与血凝块溶解相关的潜在有害事件。
Pretreatment with a low dose of thrombin reduces brain edema after both hemorrhagic and ischemic stroke. We call this phenomenon thrombin preconditioning (TPC) or thrombin-induced brain tolerance. The present study examines whether TPC can attenuate the brain edema induced by lysed red blood cells (RBCs) to determine whether thrombin production early in an intracerebral hemorrhage (ICH) might alter potentially injurious events associated with clot resolution. It also examines whether TPC might be protective by altering iron handling within the brain, particularly through modulating transferrin (Tf) and transferrin receptor (TfR) levels. Brain edema was measured by wet/dry weight. Western blot analysis and immunohistochemistry were used for Tf and TfR measurements. We found that TPC reduces lysed RBC-induced brain edema and upregulates both Tf and TfR levels in the brain. Thrombin formation after an ICH may be part of a signaling cascade that acts to limit potentially injurious events associated with clot resolution through altering iron-handling proteins.