Regulated DNA rearrangement during sporulation in Bacillus weihenstephanensis KBAB4
Regulated DNA rearrangement during sporulation in Bacillus weihenstephanensis KBAB4
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DOI：
10.1111/mmi.12375
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发表时间：
2013-10-01
影响因子：
3.6
通讯作者：
Sato, Tsutomu
中科院分区：
文献类型：
--
作者：
Abe, Kimihiro;Yoshinari, Akira;Sato, Tsutomu
温带噬菌体可以将其基因组整合到宿主染色体的特定区域以产生溶源（原噬菌体）。在基因组插入过程中，原噬菌体可能会中断基因编码序列。在枯草芽孢杆菌中，sigma 因子基因 sigK 被 48kb 原噬菌体样元件中断。 sigK 是孢子形成期间两个部分基因的复合编码序列。然而，二十多年来，除了 sigK 之外，在孢子形成体中还没有发现更多 DNA 元件介导的基因重建的例子。在这里，我们报道了 B.weihenstephanensisKBAB4 中吡啶二羧酸 (DPA) 合成酶亚基 spoVFB 的基因被名为 vfbin 的类原噬菌体元件中断。 DPA 在母细胞中合成，是维持孢子休眠所必需的。我们发现 spoVFB 是通过切除 vfbin 的染色体重排在母细胞中产生的复合编码序列。此外，vfbin 在噬菌体诱导剂处理后引起切除，但 vfbin 作为原噬菌体似乎有缺陷。我们还发现了各种孢子形成细菌，其中与孢子形成相关的基因被原噬菌体样 DNA 元件破坏。这些结果证明了影响 sigK 以外的孢子形成基因的类似机制的第一个例子，并表明这种原噬菌体介导的 DNA 重排是孢子形成细菌中的常见现象。
Temperate phages can integrate their genomes into a specific region of a host chromosome to produce lysogens (prophage). During genome insertion, prophages may interrupt the gene coding sequence. In Bacillus subtilis, the sigma factor gene sigK is interrupted by a 48kb prophage-like element. sigK is a composite coding sequence from two partial genes during sporulation. For over two decades, however, no further examples of DNA element-mediated gene reconstitution other than sigK have been identified in spore formers. Here we report that the gene for dipicolinic acid (DPA) synthetase subunit spoVFB in B.weihenstephanensisKBAB4 is interrupted by a prophage-like element named vfbin. DPA is synthesized in the mother cell and required for maintaining spore dormancy. We found that spoVFB was a composite coding sequence generated in the mother cell via chromosomal rearrangement that excised vfbin. Furthermore, vfbin caused excision after phage-inducer treatment, but vfbin appeared to be defective as a prophage. We also found various spore-forming bacteria in which sporulation-related genes were disrupted by prophage-like DNA elements. These results demonstrate the first example of a similar mechanism that affects a sporulation gene other than sigK and suggest that this prophage-mediated DNA rearrangement is a common phenomenon in spore-forming bacteria.