Ciguatera incidence and fish toxicity in Okinawa, Japan
Ciguatera incidence and fish toxicity in Okinawa, Japan
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DOI：
10.1016/j.toxicon.2009.05.036
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发表时间：
2010-10-01
期刊：
影响因子：
2.8
通讯作者：
Inafuku, Yasuo
中科院分区：
文献类型：
--
作者：
Oshiro, Naomasa;Yogi, Kentaro;Inafuku, Yasuo
冲绳位于亚热带地区，是日本雪卡毒素最高的地区。据官方统计，1997年至2006年期间报告了33起疫情，涉及103名患者。涉及的物种是天花，笛鲷，笛鲷单点，棕点埃松，不明笛鲷，方棘鲷、点石鲷、多角石斑鱼、大眼鲹、海鳗。通过小鼠生物测定法确定的剩余膳食的毒性范围为0.025至0.8 MU/g或以上（相当于0.175-5.6 ng CTX 1B/g）。共采集鱼类标本612份，分属于单斑笛鲷、波哈笛鲷、银斑笛鲷、拉塞尔笛鲷、白纹天花、白纹天花、红点笛鲷、白纹天花、白纹笛鲷、从冲绳附近海域采集褐点鲀（fuscoguttatus），用小鼠生物测定法检测其毒性。有毒鱼类的检出率为：单点钩虾32.3%，单点钩虾32.3%，单点钩虾32.3%，单点钩虾32.3%. bohar为11.9%，V. lanjiang为14.3%，E.褐点绦虫占20.8%。在36个白纹伊蚊样本中，只有1个样本有毒，而在74个鲁氏乳杆菌样本中，只有2个样本有毒。35份银色斑螯蟹样品均未检出毒性。重量小于4公斤的L bohar样品也没有毒性。在所有毒性样品中，通过LC/MS分析检测到CTX 1B，但未检测到CTX 3C和51-羟基CTX 3C。(C)2009爱思唯尔有限公司保留所有权利。
Okinawa being located in the subtropical region has the highest incidence of ciguatera in Japan. Officially, 33 outbreaks involving 103 patients have been reported between 1997 and 2006. The implicated species were Variola louti, Lutjanus bohar, Lutjanus monostigma, Epinephelus fuscoguttatus, unidentified Lutjanus sp., Plectropomus areolatus, Oplegnathus punctatus, Epinephelus polyphekadion, Caranx ignobilis and moray eel. Toxicities of the leftover meals, as determined by mouse bioassays, ranged from 0.025 to 0.8 MU/g or above (equivalent to 0.175-5.6 ng CTX1B/g). We collected 612 specimens of fish belonging to L monostigma, L bohar, Lutjanus argentimaculatus, Lutjanus russellii, V. louti, Variola albimarginata, and E. fuscoguttatus from the coasts around Okinawa and examined the toxicity of the flesh by the mouse bioassay. The rate of toxic fish was as follows: L monostigma: 32.3%, L. bohar: 11.9%, V. louti: 14.3%, E. fuscoguttatus: 20.8%. Only one out of 36 samples of V. albimarginata and two of 74 samples of L russellii were found toxic. None of the 35 samples of L argentimaculatus was toxic. Nor the L bohar samples weighing less than 4 kg were toxic. In all toxic samples, CTX1B was detected by LC/MS analysis but CTX3C and 51-hydroxyCTX3C were not. (C) 2009 Elsevier Ltd. All rights reserved.