Automated HPLC assay for urinary collagen cross-links: Effect of age, menopause, and metabolic bone diseases
Automated HPLC assay for urinary collagen cross-links: Effect of age, menopause, and metabolic bone diseases
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DOI：
10.1373/clinchem.2008.105262
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发表时间：
2008-09-01
影响因子：
9.3
通讯作者：
Steinmann, Beat
中科院分区：
文献类型：
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作者：
Kraenzlin, Marius E.;Kraenzlin, Claude A.;Steinmann, Beat
背景：吡啶鎓交联吡啶啉（PYD）和脱氧吡啶啉（DPD）是公认的骨吸收标志物。我们评估了市售PYD HPLC测定的分析和临床性能，并建立了儿童和成人的参考区间。我们用了一套市售的试剂（Chromsystems Instruments & Chemicals）测量319名健康对照者的PYD和DPD（156名绝经前女性，80名健康男性和83名1个月至14岁的健康儿童）和397名代谢性骨病患者（绝经后骨质疏松症，n = 175;男性骨质疏松症，n = 176;甲状旁腺功能亢进，n = 17;甲状腺功能亢进，n = 19;佩吉特病，n = 10）。
BACKGROUND: The pyridinium cross-links pyridinoline (PYD) and deoxypyridinoline (DPD) are established markers of bone resorption. We evaluated the analytical and clinical performance of a commercially available PYD HPLC assay and established reference intervals in children and adults.METHODS: We used a commercially available reagent set (Chromsystems Instruments & Chemicals) to measure PYD and DPD in 319 healthy controls (156 premenopausal women, 80 healthy men, and 83 healthy children age 1 month to 14 years) and 397 patients with metabolic bone diseases (postmenopausal osteoporosis, n = 175; male osteoporosis, n = 176; hyperparathyroidism, n = 17; hyperthyroidism, n = 19; Paget disease, n = 10).RESULTS: The mean intraassay and interassay CVs were