Stable accumulation of seed storage proteins containing vaccine peptides in transgenic soybean seeds
Stable accumulation of seed storage proteins containing vaccine peptides in transgenic soybean seeds
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DOI：
10.1016/j.jbiosc.2014.04.004
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发表时间：
2014-10-01
影响因子：
2.8
通讯作者：
Terakawa, Teruhiko
中科院分区：
文献类型：
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作者：
Maruyama, Nobuyuki;Fujiwara, Keigo;Terakawa, Teruhiko
转基因植物用于大规模生产药物和工业蛋白质的使用显著增加。在这里，我们报告了转基因大豆种子中含有疾病疫苗肽的种子贮藏蛋白的稳定积累。为了在大豆种子中合成疫苗肽，我们使用种子贮藏蛋白作为载体，并使用缺乏主要种子贮藏蛋白的大豆育种系作为宿主。将疫苗肽插入A1aB1b亚基三维结构中的柔性无序区域。在无序区域中含有A1aB1b亚基的疫苗肽被分选到蛋白质储存空泡中，其中疫苗肽被蛋白酶部分切割。相比之下，含有疫苗肽的A1 aB1 b亚基的内质网（ER）保留型在源自ER的隔室中以完整的前体形式积累。这些结果表明，内质网可能是一个细胞器，以种子贮藏蛋白为载体的生物活性肽的稳定积累。(c)2014年，生物技术学会，日本。All rights reserved.
There has been a significant increase in the use of transgenic plants for the large-scale production of pharmaceuticals and industrial proteins. Here, we report the stable accumulation of seed storage proteins containing disease vaccine peptides in transgenic soybean seeds. To synthesize vaccine peptides in soybean seeds, we used seed storage proteins as a carrier and a soybean breeding line lacking major seed storage proteins as a host. Vaccine peptides were inserted into the flexible disordered regions in the A1aB1b subunit three-dimensional structure. The A1aB1b subunit containing vaccine peptides in the disordered regions were sorted to the protein storage vacuoles where vaccine peptides are partially cleaved by proteases. In contrast, the endoplasmic reticulum (ER)-retention type of the A1aB1b subunit containing vaccine peptides accumulated in compartments that originated from the ER as an intact pro-form. These results indicate that the ER may be an organelle suitable for the stable accumulation of bioactive peptides using seed storage proteins as carriers. (c) 2014, The Society for Biotechnology, Japan. All rights reserved.