Antimicrobial activity and mechanism of the human milk-sourced peptide Casein201
Antimicrobial activity and mechanism of the human milk-sourced peptide Casein201
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母乳肽Casein201的抗菌活性及机制
DOI：
10.1016/j.bbrc.2017.02.108
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发表时间：
2017
影响因子：
3.1
通讯作者：
Chen Ting
中科院分区：
文献类型：
--
作者：
Zhang Fan;Cui Xianwei;Fu Yanrong;Zhang Jun;Zhou Yahui;Sun Yazhou;Wang Xing;Li Yun;Liu Qianqi;Chen Ting
简介酪蛋白201 是一种母乳来源的肽，在早产儿和足月母亲中存在显着差异。本研究旨在论证Casein201对新生儿感染常见病原体的生物学特性、抗菌活性及作用机制。方法采用生物信息学方法对生物学特性进行分析。采用纸片扩散法和流式细胞仪检测Casein201的抗菌活性。使用酶标仪测量 Casein201 的杀伤动力学。通过电镜和电泳技术研究了Casein201的抗菌机制。结果生物信息学分析表明Casein201源自β-酪蛋白，并表现出明显的序列重叠。抗菌试验表明Casein201可抑制金黄色葡萄球菌和小肠结肠炎耶尔森氏菌的生长。超微结构分析表明Casein201的抗菌活性是通过细胞质结构的崩解和细菌细胞包膜的改变而实现的，而不是与DNA结合。结论我们总结了Casein201的抗菌活性和机制。我们的数据表明Casein201对于预防和治疗新生儿感染病原体具有潜在的治疗价值。
IntroductionCasein201 is one of the human milk sourced peptides that differed significantly in preterm and full-term mothers. This study is designed to demonstrate the biological characteristics, antibacterial activity and mechanisms of Casein201 against common pathogens in neonatal infection.MethodologyThe analysis of biological characteristics was done by bioinformatics. Disk diffusion method and flow cytometry were used to detect the antimicrobial activity of Casein201. Killing kinetics of Casein201 was measured using microplate reader. The antimicrobial mechanism of Casein201 was studied by electron microscopy and electrophoresis.ResultsBioinformatics analysis indicates that Casein201 derived from β-casein and showed significant sequence overlap. Antibacterial assays showed Casein201 inhibited the growth ofS taphylococcusaureus andY ersinia enterocolitica. Ultrastructural analyses revealed that the antibacterial activity of Casein201 is through cytoplasmic structures disintegration and bacterial cell envelope alterations but not combination with DNA.ConclusionWe conclude the antimicrobial activity and mechanism of Casein201. Our data demonstrate that Casein201 has potential therapeutic value for the prevention and treatment of pathogens in neonatal infection.