Transplanted mesenchymal stem cells are effective for skin regeneration in acute cutaneous wounds
Transplanted mesenchymal stem cells are effective for skin regeneration in acute cutaneous wounds
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DOI：
10.3727/000000004783983765
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发表时间：
2004-01-01
影响因子：
3.3
通讯作者：
Ishii, T
中科院分区：
文献类型：
--
作者：
Satoh, H;Kishi, K;Ishii, T
间充质干细胞（MSC）具有响应于不同器官提供的线索而进行细胞类型的位点特异性分化的能力。这种现象表明，MSC参与皮肤伤口再生。然而，目前还没有关于局部应用MSC对皮肤伤口再生的影响的报道。为了研究间充质干细胞对伤口再生的影响，我们培养了大鼠股骨的骨髓细胞，并用分化培养基处理塑料贴壁细胞以诱导分化。经处理后，我们发现骨髓来源的可塑性贴壁细胞具有成肌、成软骨和成脂肪的能力，表明这些细胞是间充质干细胞。将骨髓来源的塑料粘附细胞皮内注射到大鼠皮肤中，并立即通过注射区域制作线性全层切口伤口。术后第14天，移植骨髓源性塑料粘附细胞的伤口已愈合，瘢痕非常细小。胶原结构厚，似乎与正常真皮相似。组织形态学分析表明，对照组和骨髓源性塑料贴壁细胞移植的伤口之间存在显着差异。这些结果表明，移植的MSC可以非常正常地响应伤口愈合和再生真皮结构。
Mesenchymal stem cells (MSCs) possess the capacity for site-specific differentiation of cell types in response to cues provided by different organs. This phenomenon suggests that MSCs participate in cutaneous wound regeneration. However, there are no prior reports on the influence of the local application of MSCs on cutaneous wound regeneration. To examine the effects of MSCs on wound regeneration, we cultured bone marrow cells of the femur of rats and treated the plastic adherent cells with a differentiation medium to induce differentiation. After treatment, we found that the bone marrow-derived plastic adherent cells possessed myogenesis, chondrogenesis, and adipogenesis capabilities, indicating that these cells are MSCs. The bone marrow-derived plastic adherent cells were injected intradermally into the skin of rats, and linear full-thickness incisional wounds were made immediately through the injected area. At 14 days after operation, wounds transplanted with bone marrow-derived plastic adherent cells had healed with very fine scars. Collagen architecture was thick and appeared to be similar to normal dermis. Histomorphologic scale analysis demonstrated significant differences between the control and the wounds transplanted with bone marrow-derived plastic adherent cells. These results indicate that transplanted MSCs can respond quite normally to wound healing and regenerate dermal structure.