SEVERAL DISTINCT TYPES OF SEQUENCE ELEMENTS ARE REQUIRED FOR EFFICIENT MESSENGER-RNA 3' END FORMATION IN A PEA RBCS GENE
SEVERAL DISTINCT TYPES OF SEQUENCE ELEMENTS ARE REQUIRED FOR EFFICIENT MESSENGER-RNA 3' END FORMATION IN A PEA RBCS GENE
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DOI：
10.1128/mcb.12.12.5406
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发表时间：
1992-12-01
影响因子：
5.3
通讯作者：
HUNT, AG
中科院分区：
文献类型：
--
作者：
MOGEN, BD;MACDONALD, MH;HUNT, AG
我们已经进行了广泛的接头置换分析的多聚腺苷酸化信号从豌豆rbcS基因。从这些研究中，我们可以确定至少两个，也许三个，不同的类的顺式元件参与mRNA的3'端形成在这个基因。其中之一，被称为远上游元件，位于60和120核苷酸上游从其相关的多聚腺苷酸化位点，似乎主要是由一系列的UG基序。第二个，称为近上游元件，更接近多聚腺苷酸位点，可能在功能上类似于哺乳动物多聚腺苷酸化信号AAUAAA，即使实际涉及的序列可能不是AAUAAA。第三种可能的类型是假定的切割和多聚腺苷酸化位点本身。我们发现rbcS-E9远上游元件可以取代另一种植物多聚腺苷酸化信号中的类似元件，即花椰菜花叶病毒，并且一个近上游元件可以与两个poly（A）位点中的任一个起作用。因此，这些不同的顺式元件在很大程度上是可互换的。我们的研究表明，一个细胞的植物基因具有上游元件不同AAUAAA参与mRNA的3'端形成，植物基因可能有模块化，多组分聚腺苷酸化信号。
We have conducted an extensive linker substitution analysis of the polyadenylation signal from a pea rbcS gene. From these studies, we can identify at least two, and perhaps three, distinct classes of cis element involved in mRNA 3' end formation in this gene. One of these, termed the far-upstream element, is located between 60 and 120 nt upstream from its associated polyadenylation sites and appears to be largely composed of a series of UG motifs. A second, termed the near-upstream element, is more proximate to poly(A) sites and may be functionally analogous to the mammalian polyadenylation signal AAUAAA, even though the actual sequences involved may not be AAUAAA. The third possible class is the putative cleavage and polyadenylation site itself. We find that the rbcS-E9 far-upstream element can replace the analogous element in another plant polyadenylation signal, that from cauliflower mosaic virus, and that one near-upstream element can function with either of two poly(A) sites. Thus, these different cis elements are largely interchangeable. Our studies indicate that a cellular plant gene possesses upstream elements distinct from AAUAAA that are involved in mRNA 3' end formation and that plant genes probably have modular, multicomponent polyadenylation signals.