AN IMMUNOCYTOCHEMICAL INVESTIGATION WITH MONOCLONAL-ANTIBODIES TO SOMATOSTATIN
AN IMMUNOCYTOCHEMICAL INVESTIGATION WITH MONOCLONAL-ANTIBODIES TO SOMATOSTATIN
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DOI：
10.1007/bf00495150
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发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
BROWN, JC
中科院分区：
文献类型：
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作者：
BUCHAN, AMJ;SIKORA, LKJ;BROWN, JC
四个生长抑素特异性单克隆抗体已生产和特点。这些抗体用于评估人、狒狒和大鼠胰腺和胃肠道中含生长抑素细胞与其他10种含肽内分泌细胞的解剖关系。所研究的肽是胃泌素、胆囊收缩素、胃动素、促胰液素、神经降压素、胃抑制多肽、肠胰高血糖素、胰高血糖素、胰多肽和胰岛素。生长抑素细胞与另一种内分泌细胞紧密接触的唯一区域是在胰腺和胃窦。在胰腺生长抑素细胞通常被认为是密切接触胰岛素，胰高血糖素和胰腺多肽细胞和罕见的接触是证明与胃泌素免疫反应细胞在胃窦的大鼠和man.In所有其他情况下，没有证据表明生长抑素细胞和其他肠内分泌细胞之间的密切解剖关系。
Four monoclonal antibodies specific for somatostatin have been produced and characterized. These antibodies were used to assess the anatomical relationship of somatostatin-containing cells in the pancreas and gastrointestinal tract of man, baboon and rat with ten other peptide-containing endocrine cells. The peptides investigated were gastrin, cholecystokinin, motilin, secretin, neurotensin, gastric inhibitory polypeptide, gut-glucagon, pancreatic glucagon, pancreatic polypeptide and insulin. The only regions in which somatostatin cells were seen in close contact with another endocrine cell were in the pancrease and the gastric antrum. In the pancreas somatostatin cells were commonly seen in close contact with insulin, glucagon and pancreatic polypeptide cells and infrequent contact was demonstrable with the gastrin-immunoreactive cells in the antrum of both rat and man. In all other cases no evidence was obtained for a close anatomical relationship between somatostatin cells and the other enteroendocrine cells.