Derivation of Porcine Extra-Embryonic Endoderm Cell Lines Reveals Distinct Signaling Pathway and Multipotency States.
Derivation of Porcine Extra-Embryonic Endoderm Cell Lines Reveals Distinct Signaling Pathway and Multipotency States.
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DOI：
10.3390/ijms222312918
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发表时间：
2021-11-29
影响因子：
5.6
通讯作者：
Li RF
中科院分区：
文献类型：
--
作者：
Zhang ML;Jin Y;Zhao LH;Zhang J;Zhou M;Li MS;Yin ZB;Wang ZX;Zhao LX;Li XH;Li RF
植入前胚泡的内细胞团由上胚层和下胚层组成，胚胎干细胞（ESC）和胚胎外内胚层（XEN）干细胞可分别从上胚层和下胚层衍生。重要的是，每种干细胞类型都保留了其体内组织来源的定义特性和谱系限制。我们已经开发了一种新的方法来获得猪XEN（pXEN）细胞，通过培养囊胚与化学鸡尾酒培养系统。pXEN细胞对XEN标志物呈阳性，包括Gata 4、Gata 6、Sox 17和Sall 4，但对多能标志物Oct 4、Sox 2和Nanog不呈阳性。pXEN细胞还保留了在体外经历内脏内胚层（VE）和壁内胚层（PE）分化的能力。pXEN的维持需要FGF/MEK+TGFβ信号通路。pXEN细胞在50代以上的培养中表现出稳定的表型，并且可以从囊胚重复建立或从我们实验室建立的幼稚样ESC转化。这些细胞为探索猪胚胎发育和分化的途径提供了新的工具，并为建立具有种系嵌合和配子发育潜能的猪胚胎干细胞提供了进一步的参考。
The inner cell mass of the pre-implantation blastocyst consists of the epiblast and hypoblast from which embryonic stem cells (ESCs) and extra-embryonic endoderm (XEN) stem cells, respectively, can be derived. Importantly, each stem cell type retains the defining properties and lineage restriction of its in vivo tissue origin. We have developed a novel approach for deriving porcine XEN (pXEN) cells via culturing the blastocysts with a chemical cocktail culture system. The pXEN cells were positive for XEN markers, including Gata4, Gata6, Sox17, and Sall4, but not for pluripotent markers Oct4, Sox2, and Nanog. The pXEN cells also retained the ability to undergo visceral endoderm (VE) and parietal endoderm (PE) differentiation in vitro. The maintenance of pXEN required FGF/MEK+TGFβ signaling pathways. The pXEN cells showed a stable phenotype through more than 50 passages in culture and could be established repeatedly from blastocysts or converted from the naïve-like ESCs established in our lab. These cells provide a new tool for exploring the pathways of porcine embryo development and differentiation and providing further reference to the establishment of porcine ESCs with potency of germline chimerism and gamete development.
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影响因子：
14.8
作者：
Niakan, Kathy K.;Schrode, Nadine;Cho, Lily T. Y.;Hadjantonakis, Anna-Katerina
通讯作者：
Hadjantonakis, Anna-Katerina
影响因子：
11.8
作者：
Chazaud, Claire;Yamanaka, Yojiro;Rossant, Janet
通讯作者：
Rossant, Janet
影响因子：
4.6
作者：
Mesnard, Daniel;Guzman-Ayala, Marcela;Constam, Daniel B.
通讯作者：
Constam, Daniel B.
DOI：
10.1007/s11626-020-00448-3
发表时间：
2020-05-19
影响因子：
2.1
作者：
Chen，Qiaoyu;Zhang，Hong;Li，Rongfeng
通讯作者：
Li，Rongfeng
影响因子：
11.8
作者：
Guo, Guoji;Huss, Mikael;Robson, Paul
通讯作者：
Robson, Paul