Nonsense mutants defining seven new genes of the lipid-containing bacteriophage PR4.
Nonsense mutants defining seven new genes of the lipid-containing bacteriophage PR4.
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无义突变体定义了含脂质噬菌体 PR4 的七个新基因。
DOI：
10.1016/0042-6822(90)90455-z
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发表时间：
1990
期刊：
影响因子：
3.7
通讯作者：
CronanJr,JE
中科院分区：
文献类型：
--
作者：
VandenBoom,T;CronanJr,JE
分离出38个新的含脂质噬菌体PR 4的无义突变体。这些突变体定义了七个新的病毒基因，包括编码末端基因组蛋白和辅助裂解因子的基因。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）鉴定了在紫外线照射的细胞中产生的有缺陷的基因产物，所述细胞用来自每个新的遗传组的代表性突变体感染。用无义突变体感染的紫外线照射细胞的提取物也缺乏两种较低分子量的蛋白质，所述无义突变体产生缺陷的主要衣壳蛋白P2。在用两个独立的主要衣壳蛋白突变体的一步回复突变体感染的细胞中恢复所有三种蛋白质的合成，这表明翻译后加工或重叠基因的可能性。使用SDS-PAGE检查野生型PR 4感染的细胞中蛋白质合成的时间过程。这些分析揭示了在噬菌体PR4感染后产生的至少34种蛋白质，其不存在于未感染的对照培养物中。
Thirty-eight new nonsense mutants of the lipid-containing bacteriophage PR4 were isolated. These mutants define seven new viral genes, including the gene encoding the terminal genome protein and an accessory lytic factor. The defective gene products produced in uv-irradiated cells infected with representative mutants from each of the new genetic groups were identified using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Extracts of uv-irradiated cells infected with nonsense mutants that produce a defective major capsid protein, P2, also lacked two lower molecular weight proteins. The synthesis of all three protein species was recovered in cells infected with one-step revertants of two independent major capsid protein mutants, suggesting the possibility of post-translational processing or overlapping genes. The time course of protein synthesis in wild-type PR4-infected cells was examined using SDS-PAGE. These analyses revealed at least 34 proteins produced following phage PR4 infection that were not present in uninfected control cultures.