The Mucilage Proteome of Maize (Zea mays L.) Primary Roots
The Mucilage Proteome of Maize (Zea mays L.) Primary Roots
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DOI：
10.1021/pr901168v
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发表时间：
2010-06-01
影响因子：
4.4
通讯作者：
Li, Chunjian
中科院分区：
文献类型：
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作者：
Ma, Wei;Muthreich, Nils;Li, Chunjian
玉米（Zea mays L.）根冠细胞通过被称为粘液的无定形凝胶结构将包括蛋白质在内的多种化合物分泌到根际。本研究在无菌条件下收集了3-4天龄玉米幼苗初生根分泌的粘液，并对其组成型分泌蛋白质组进行了分析。通过nanoLC-MS/MS共鉴定了2848种不同的细胞外蛋白质。其中，代谢蛋白质（类似于25%）代表了最大类别的注释蛋白质。涉及细胞壁代谢、活性氧清除、应激反应或养分获取的综合蛋白质组提供了根-土界面所需功能的详细见解。对于先前在双子叶植物物种豌豆、拟南芥和油菜籽的相对小的数据集中鉴定的85-94%的粘液蛋白，在单子叶植物模式植物玉米的粘液蛋白质组中鉴定了一个密切的同源物，这表明单子叶植物和双子叶植物粘液蛋白质组之间具有相当程度的保守性。同源物的核心集12玉米蛋白质，包括三个超氧化物歧化酶和四个几丁质酶，提供保护免受真菌感染，目前在所有三个粘液蛋白质组研究到目前为止，在双子叶植物物种拟南芥，油菜籽，豌豆，因此可能是特别重要的。
Maize (Zea mays L.) root cap cells secrete a large variety of compounds including proteins via an amorphous gel structure called mucilage into the rhizosphere. In the present study, mucilage secreted by primary roots of 3-4 day old maize seedlings was collected under axenic conditions, and the constitutively secreted proteome was analyzed. A total of 2848 distinct extracellular proteins were identified by nanoLC-MS/MS. Among those, metabolic proteins (similar to 25%) represented the largest class of annotated proteins. Comprehensive sets of proteins involved in cell wall metabolism, scavenging of reactive oxygen species, stress response, or nutrient acquisition provided detailed insights in functions required at the root-soil interface. For 85-94% of the mucilage proteins previously identified in the relatively small data sets of the dicot species pea, Arabidopsis, and rapeseed, a close homologue was identified in the mucilage proteome of the monocot model plant maize, suggesting a considerable degree of conservation between mono and dicot mucilage proteomes. Homologues of a core set of 12 maize proteins including three superoxide dismutases and four chitinases, which provide protection from fungal infections, were present in all three mucilage proteomes investigated thus far in the dicot species Arabidopsis, rapeseed, and pea and might therefore be of particular importance.