Effects of cytosine arabinoside, daunomycin, mithramycin, azacytidine, adriamycin, and camptothecin on mammalian cell cycle traverse.
Effects of cytosine arabinoside, daunomycin, mithramycin, azacytidine, adriamycin, and camptothecin on mammalian cell cycle traverse.
复制标题
胞嘧啶阿拉伯糖苷、道诺霉素、光神霉素、氮胞苷、阿霉素和喜树碱对哺乳动物细胞周期遍历的影响。
作者：
R. Tobey
在同步培养的中国仓鼠细胞中，研究了6种化疗药物对哺乳动物细胞周期穿越的影响。阿糖苷胞嘧啶抑制DNA合成，并显著降低细胞从G1期进入S期的速度。在低剂量下，道诺霉素和阿霉素对细胞进入S期只有轻微的影响，但它们几乎完全有效地阻止细胞进入有丝分裂，5-阿扎胞苷极大地抑制细胞进入S期。然而，在加入阿扎胞苷时已经处于S中的细胞继续合成DNA，但不能进行有丝分裂。米霉素几乎不影响S的进入，而G2的完成被部分抑制，尽管在米霉素处理的细胞中G2过程的敏感性远不如用道诺霉素或阿霉素处理的细胞明显。喜树碱允许DNA合成的起始，但它有效地阻止了细胞在试验方案中规定的潜伏期内进行有丝分裂。
Summary Six chemotherapeutic agents were tested for effects on mammalian cell cycle traverse in synchronized cultures of Chinese hamster cells. Cytosine arabinoside was found to inhibit DNA synthesis, as well as to reduce grossly the rate of progression from G1 into S. At low dosage levels, daunomycin and adriamycin had only a slight effect upon entry of cells into S, but they were almost totally effective in preventing cells from reaching mitosis, and 5-azacytidine greatly inhibited progression into S phase. However, cells already in S at the time azacytidine was added continued to synthesize DNA but were unable to progress to mitosis. Entry into S was hardly affected by mithramycin, while completion of G2 was partially inhibited, although the sensitivity of G2 processes was much less pronounced in mithramycin-treated cells than in cells treated with daunomycin or adriamycin. Camptothecin allowed initiation of DNA synthesis, but it effectively prevented cells from progressing to mitosis in the incubation period allotted in the test protocol.