Expression of Rab27B-binding protein Slp1 in pancreatic acinar cells and its involvement in amylase secretion
Expression of Rab27B-binding protein Slp1 in pancreatic acinar cells and its involvement in amylase secretion
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DOI：
10.1016/j.abb.2008.04.031
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发表时间：
2008-07-01
影响因子：
3.9
通讯作者：
Fukuda, Mitsunori
中科院分区：
文献类型：
--
作者：
Saegusa, Chika;Kanno, Eiko;Fukuda, Mitsunori
SLp1是一种可能的Rab27效应蛋白，与细胞膜转运有关；然而，SLp1蛋白的确切组织分布和功能在很大程度上仍不清楚。在本研究中，我们研究了SLP1在小鼠体内的组织分布，发现SLP1在胰腺中大量表达，尤其是在胰腺腺泡细胞的顶端区域。SLP1与Rab27B在体内相互作用，两种蛋白共定位于酶原颗粒上。对禁食的SLp1基因敲除小鼠的形态分析显示，胰腺腺泡细胞中的酶原颗粒数量增加，表明SLp1是胰腺外分泌淀粉酶机制的一部分。(C)2008 Elsevier Inc.保留所有权利。
Slp1 is a putative Rab27 effector protein and implicated in intracellular membrane transport; however, the precise tissue distribution and function of Slp1 protein remain largely unknown. In this study we investigated the tissue distribution of Slp1 in mice and found that Slp1 is abundantly expressed in the pancreas, especially in the apical region of pancreatic acinar cells. Slp1 interacted with Rab27B in vivo and both proteins were co-localized on zymogen granules. Morphological analysis of fasted Slp1 knockout mice showed an increased number of zymogen granules in the pancreatic acinar cells, indicating that Slp1 is part of the machinery of amylase secretion by the exocrine pancreas. (C) 2008 Elsevier Inc. All rights reserved.