Plasmid-borne armA methylase gene, together with blaCTX-M-15 and blaTEM-1, in a Klebsiella oxytoca isolate from China.
Plasmid-borne armA methylase gene, together with blaCTX-M-15 and blaTEM-1, in a Klebsiella oxytoca isolate from China.
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DOI：
10.1099/jmm.0.2008/001271-0
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发表时间：
2008-10
影响因子：
3
通讯作者：
Ying Zhang;Hua Zhou;Xiaoxiao Shen;P. Shen;Yunsong Yu;Lan-juan Li
中科院分区：
文献类型：
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作者：
Ying Zhang;Hua Zhou;Xiaoxiao Shen;P. Shen;Yunsong Yu;Lan-juan Li
从中国分离的447株产超广谱β-内酰胺酶的肠杆菌科细菌中筛选出一株产超广谱β-内酰胺酶的产氨酸克雷伯菌157株。催产克雷伯菌157对氨基糖苷类、环丙沙星和大多数β-内酰胺类抗生素耐药。对氨基糖苷类和β-内酰胺类抗生素的耐药性可通过接合转移到受体大肠杆菌。在催产克雷伯氏菌157和接合菌株600(PEC157)中检测到ARMA、blaCTX-M-15和blaTEM1基因。在催产克雷伯氏菌157中发现了GyrA基因的aa87突变。从产酸克雷伯氏菌157(PKO157)和大肠杆菌600(PEC157)中提取了一个约55kb的质粒。Southern印迹杂交证实，ARMA、blaCTX-M-15和blaTEM1基因均位于pEC157上。同时，对ARMA的遗传环境进行了研究。在ARMA基因两侧发现了与之前在不同国家分离的大肠杆菌、肺炎克雷伯菌和弗劳地柠檬酸杆菌中报道的相同的转座元件。
An armA-producing Klebsiella oxytoca isolate, strain 157, was detected after screening of 447 extended-spectrum beta-lactamase-producing Enterobacteriaceae isolates in China. K. oxytoca 157 was resistant to aminoglycosides, ciprofloxacin and most beta-lactams. Resistance to aminoglycosides and beta-lactams could be transferred to recipient Escherichia coli by conjugation. armA, blaCTX-M-15 and blaTEM-1 genes were detected in K. oxytoca 157 and transconjugant E. coli strain 600(pEC157). Mutation of aa 87 in GyrA was found in K. oxytoca 157. A plasmid of approximately 55 kb was extracted from K. oxytoca 157(pKO157) and E. coli 600(pEC157). Southern blot hybridization confirmed that the armA, blaCTX-M-15 and blaTEM-1 genes were all located on this conjugative plasmid (pEC157). PCR mapping was also performed to investigate the genetic environment of armA. The armA gene was found to be flanked by the same putative transposable elements as reported previously in E. coli, Klebsiella pneumoniae and Citrobacter freundii isolates from different countries.