Beta-endorphin is present in the male reproductive tract of five species.
Beta-endorphin is present in the male reproductive tract of five species.
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β-内啡肽存在于五个物种的雄性生殖道中。
DOI：
10.1095/biolreprod27.3.755
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发表时间：
1982
影响因子：
3.6
通讯作者：
Bardin,CW
中科院分区：
文献类型：
--
作者：
Shu-Dong,T;Phillips,DM;Halmi,N;Krieger,D;Bardin,CW
本实验室先前的研究已经证实，大鼠的附睾、精囊和输精管的间质细胞和上皮细胞中存在免疫染色的β-内啡肽样物质（β-EP）。如果能证明它们不是大鼠的特性，这些观察结果就会得到加强。因此，我们现在提出免疫细胞化学证据，证明β- ep存在于小鼠、仓鼠、豚鼠和兔的间质细胞中。在所有被检物种的支持细胞、肌样细胞、内皮细胞和生殖细胞中均未发现免疫反应性物质。小鼠、豚鼠、家兔和大鼠附睾均可见免疫染色的β- ep，但仓鼠未见。小鼠和大鼠的输精管和精囊的上皮细胞中也存在免疫染色物质，这是迄今为止唯一检测到的两个物种。由于β- ep存在于间质细胞中，我们想知道这种肽是否可以在其他类固醇产生组织中鉴定出来。当大鼠卵巢和肾上腺与抗β内啡肽反应时，黄体和肾上腺皮质出现染色。肾上腺髓质及卵巢其他部位未见染色。为了确定睾丸和附睾中检测到的β- ep是否来源于垂体，我们在2周后切除动物的垂体并检查组织。间质细胞和附睾上皮均保持免疫染色。综上所述，免疫染色的β- ep已在五种间质细胞中被鉴定出来。在男性生殖道其他部位的上皮以及卵巢和肾上腺的类固醇分泌细胞中也存在染色物质。这种β- ep可能在睾丸和男性生殖道的其他部分具有旁分泌功能。
Previous studies from this laboratory have demonstrated immunostainableβ-endorphin-like material (β-EP) in Leydig cells and epithelia of the epididymis, seminal vesicle and vas deferens of the rat. These observations would be strengthened if it could be demonstrated that they were not a peculiarity of the rat. Accordingly, we now present immunocytochemical evidence for the presence ofβ-EP in the Leydig cells of mouse, hamster, guinea pig and rabbit. No immunoreactive material was identified in Sertoli, myoid, endothelial or germ cells of any of the species examined. Immunostainableβ-EP was also demonstrated in the epididymides of mouse, guinea pig, rabbit, and rat, but not hamster. Immunostainable material was also present in the epithelia of the vas deferens and seminal vesicles of mouse and rat, the only two species thus far examined. Sinceβ-EP was present in Leydig cells, we wondered whether this peptide could be identified in other steroid-producing tissues. When rat ovaries and adrenals were reacted with anti-βendorphin, staining was demonstrated in corpus luteum and adrenal cortex. No staining was observed in the adrenal medulla or other portions of the ovary. In order to determine whether theβ-EP detected in the testis and epididymis was derived from a pituitary source, animals were hypophysectomized and tissues examined 2 weeks later. Both the Leydig cells and the epididymal epithelium remained immunostainable. In summary, immunostainableβ-EP has been identified in Leydig cells of five species. Stainable material is also present in the epithelium of other portions of the male reproductive tract and in steroid-secreting cells of the ovary and the adrenal. Suchβ-EP may have a paracrine function in the testis and other portions of the male reproductive tract.