Molecular epidemiology of Rickettsia sp. and Coxiella burnetii collected from Hyalomma asiaticum in Bactrian camels (Camelus bactrianus) in inner Mongolia of China
Molecular epidemiology of Rickettsia sp. and Coxiella burnetii collected from Hyalomma asiaticum in Bactrian camels (Camelus bactrianus) in inner Mongolia of China
复制标题
立克次体的分子流行病学。
DOI：
10.1016/j.ttbdis.2020.101548
复制
发表时间：
2020-11-01
影响因子：
3.2
通讯作者：
Geri, Letu
中科院分区：
文献类型：
--
作者：
Batu, Naren;Wang, Yunchong;Geri, Letu
本研究的目的是确定中国内蒙古双峰驼亚洲璃眼蜱中是否存在立克次体和贝氏柯克斯体。共385个H。对2017 - 2018年采集自内蒙古阿拉善地区双峰驼的亚洲驼成虫进行了PCR结合测序检测。结果表明，立克次体和衣原体阳性分别为18份（4.7%）和5份（1.3%）. burnetii。与立克次体的17 kD和ompB基因序列及C.伯内特氏菌两株代表性的立克次体IMA-1株和C. Burnetii IMA-1菌株-用于随后的分析。NCBI BLAST和系统发育分析表明，IMA-1可能代表斑点热群立克次体的一个新种。梭贝氏柯克斯体IMA-1菌株与贝氏柯克斯体R5 A439（GenBank：CP 040059.1）归为一类。此外，在28 ℃孵育的Vero细胞中，从蜱中成功分离出立克次体。这些结果表明，H。从内蒙古双峰驼上采集的亚洲立克次体携带SFG立克次体种和C. burnetii，并可能有助于动物和人类发热性疾病的病因。
The purpose of the present study was to determine the presence of Rickettsia sp. and Coxiella burnetii in Hyalomma asiaticum collected from Bactrian camels (Camelus bactrianus) in Inner Mongolia of China. A total of 385 H. asiaticum adults collected from Bactrian camels from Alxa in Inner Mongolia from 2017 to 2018 were examined using PCR combined with sequencing. The results indicated that 18 (4.7 %) and 5 (1.3 %) samples tested positive for Rickettsia and C. burnetii, respectively. The corresponding nucleotide identities among detected genes ranged between 99.7 % and 100 % for the sequences of 17 kD and ompB from Rickettsia and 16S rRNA and FtsX from C. burnetii. Two representative strains-Rickettsia IMA-1 strain and C. burnetii IMA-1 strain-were used for subsequent analysis. NCBI BLAST and phylogenetic analyses demonstrated that the detected strain Rickettsia IMA-1 may represent a novel species of spotted fever group (SFG) rickettsiae. The C. burnetii IMA-1 strain was grouped with Coxiella burnetii str. R5A439 (GenBank: CP040059.1). In addition, the Rickettsia sp. was successfully isolated from the ticks in Vero cells incubated at 28 degrees C. These findings indicate that the H. asiaticum collected off Bactrian camels in Inner Mongolia carried SFG Rickettsia species and C. burnetii and could contribute to the etiology of febrile illness in animals and humans.