DETECTION OF AIDS VIRUS IN MACROPHAGES IN BRAIN-TISSUE FROM AIDS PATIENTS WITH ENCEPHALOPATHY
DETECTION OF AIDS VIRUS IN MACROPHAGES IN BRAIN-TISSUE FROM AIDS PATIENTS WITH ENCEPHALOPATHY
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DOI：
10.1126/science.3016903
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发表时间：
1986-09-05
期刊：
影响因子：
56.9
通讯作者：
FAUCI, AS
中科院分区：
文献类型：
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作者：
KOENIG, S;GENDELMAN, HE;FAUCI, AS
获得性免疫缺陷综合征（AIDS）患者常见的神经系统并发症之一是亚急性脑病伴进行性痴呆。通过使用病毒分离，原位杂交，免疫细胞化学和透射电子显微镜共培养技术，一个重要的细胞类型，支持艾滋病逆转录病毒在脑组织中复制的身份确定在两个受影响的个人。这些细胞是单核和多核巨噬细胞，它们在患者的大脑中活跃地合成病毒RNA并产生子代病毒体。受感染的脑巨噬细胞可作为病毒的储存库，并作为病毒在受感染宿主中传播的媒介。
One of the common neurological complications in patients with the acquired immune deficiency syndrome (AIDS) is a subacute encephalopathy with progressive dementia. By using the techniques of cocultivation for virus isolation, in situ hybridization, immunocytochemistry, and transmission electron microscopy, the identity of an important cell type that supports replication of the AIDS retrovirus in brain tissue was determined in two affected individuals. These cells were mononucleated and multinucleated macrophages that actively synthesized viral RNA and produced progeny virions in the brains of the patients. Infected brain macrophages may serve as a reservoir for virus and as a vehicle for viral dissemination in the infected host.