Ponceau S: A sensitive method for protein determination in freshly isolated and cultured cells
Ponceau S: A sensitive method for protein determination in freshly isolated and cultured cells
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Ponceau S：一种灵敏的方法，用于测定新鲜分离和培养的细胞中的蛋白质
DOI：
10.1007/bf01665914
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
H. Jauregui
中科院分区：
文献类型：
--
作者：
N. Hayner;J. Driscoll;L. Ferayorni;G. Spies;H. Jauregui
描述了一种蛋白质测定方法，其涉及同时的染料结合和蛋白质沉淀。它的灵敏度与广泛使用的Lowry和Bradford方法相当，并且没有这些方法在组织培养条件下有时会遇到的化学干扰。该测定使用容易获得的试剂，并且适用于各种细胞制备物。
A protein assay method is described that involves simultaneous dye binding and precipitation of protein. It has a sensitivity comparable to the widely used Lowry and Bradford methods without the chemical interferences sometimes encountered with these methods in tissue culture conditions. The assay uses readily available reagents and is applicable to a variety of cell preparations.