Paternal inheritance of chloroplast DNA in Douglas-fir
Paternal inheritance of chloroplast DNA in Douglas-fir
复制标题
花旗松叶绿体 DNA 的父系遗传
DOI：
10.1139/x86-205
复制
发表时间：
1986
影响因子：
2.2
通讯作者：
R. Allard
中科院分区：
文献类型：
--
作者：
D. Neale;N. Wheeler;R. Allard
研究了花旗松（Pseudotsugamenziesii（Mirb.）Franco）使用限制性片段长度多态性（RFLP）作为标记来确定。在亲本植株总DNA的SmaI和BamHI酶切位点上检测到与矮牵牛叶绿体DNA的15.3bp酶切PstI片段同源的插入/缺失序列。全同胞后代（共36个），然后在三套不同的RFLP标记的男性和女性的父母之间的杂交分析这种多态性。全同胞后代具有与父本相同的限制性片段，但有三个例外。这些数据为裸子植物叶绿体DNA的父系遗传提供了直接证据。这一结果与被子植物中叶绿体DNA严格的母系遗传和偶见的双亲遗传形成鲜明对比。这三个例外的限制性片段不同于杂交的雄性或雌性亲本，这表明某些类型的突变或重组事件已经发生。
The inheritance of chloroplast DNA in Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) was determined using a restriction fragment length polymorphism (RFLP) as a marker. An insertion/deletion sequence homologous to a 15.3 kilobase PstI fragment from petunia chloroplast DNA was detected in SmaI and BamHI digests of total DNAs of parent trees. Full-sib progeny (36 in total) were then assayed for this polymorphism in three sets of crosses between male and female parents with different RFLP markers. The full-sib progeny had the same restriction fragment as the male parent, with three exceptions. These data provide direct evidence for the paternal inheritance of chloroplast DNA in a gymnosperm. This result is in sharp contrast to the strict maternal and occasional biparental inheritance of chloroplast DNA observed in angiosperms. The three exceptions had restriction fragments unlike either the male or female parents of the cross, suggesting that some type of mutational or recombinational event had occurred d...