Meiotic Chromosome Segregation in Triploid Strains of Saccharomyces cerevisiae
Meiotic Chromosome Segregation in Triploid Strains of Saccharomyces cerevisiae
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DOI：
10.1534/genetics.110.121533
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发表时间：
2010-10-01
期刊：
影响因子：
3.3
通讯作者：
Petes, Thomas D.
中科院分区：
文献类型：
--
作者：
Charles, Jordan St.;Hamilton, Monica L.;Petes, Thomas D.
三倍体的减数分裂会产生四个高度非整倍体的配子，因为每对同源染色体的六个拷贝必须分配到四个减数分裂产物中。我们利用DNA微阵列和其他物理方法，研究了酿酒酵母（Saccharomyces cerevisiae）三倍体菌株的减数分裂染色体分离情况。在大多数具有四个可存活孢子的四分体中，两个孢子有某一同源染色体的两个拷贝，另外两个孢子只有一个拷贝。染色体随机分离到可存活的孢子中，没有产生接近单倍体或接近二倍体孢子的偏好。利用单核苷酸多态性，我们表明在大多数四分体中，所有三对同源染色体都发生了重组。从一些非整倍体孢子菌落衍生的菌株有非常高的有丝分裂染色体丢失频率，导致细胞群体在遗传上具有多样性。
Meiosis in triploids results in four highly aneuploid gametes because six copies of each homolog must be segregated into four meiotic products. Using DNA microarrays and other physical approaches, we examined meiotic chromosome segregation in triploid strains of Saccharomyces cerevisiae. In most tetrads with four viable spores, two of the spores had two copies of a given homolog and two spores had only one copy. Chromosomes segregated randomly into viable spores without preferences for generating near haploid or near diploid spores. Using single-nucleotide polymorphisms, we showed that, in most tetrads, all three pairs of homologs recombined. Strains derived from some of the aneuploid spore colonies had very high frequencies of mitotic chromosome loss, resulting in genetically diverse populations of cells.