Global gene expression analysis of peripheral blood mononuclear cells in rhesus monkey infants with CA16 infection-induced HFMD
Global gene expression analysis of peripheral blood mononuclear cells in rhesus monkey infants with CA16 infection-induced HFMD
复制标题
DOI：
10.1016/j.virusres.2016.01.002
复制
发表时间：
2016-03-01
期刊：
影响因子：
5
通讯作者：
Liu, Longding
中科院分区：
文献类型：
--
作者：
Song, Jie;Hu, Yajie;Liu, Longding
柯萨奇病毒A16(CA16)是一种主要病原体，可导致手足口病，并导致世界各地，特别是亚太地区的疫情暴发。然而，其潜在的分子机制仍不清楚。我们先前的研究已经证明，CA16的基本致病过程成功地在恒河猴婴儿身上模拟。本研究旨在研究CA16感染后不同时间恒河猴手足口病患儿外周血单个核细胞整体基因表达的变化。使用安捷伦全基因组微阵列和已建立的生物信息学工具进行全基因组表达分析。共鉴定出与细胞通讯、细胞周期、免疫系统过程、转录调控和代谢过程等5个基因本体论相关的948个差异表达基因。随后，通过Panther通路分析对免疫系统相关基因的定位，揭示了趋化因子和细胞因子信号通路以及白细胞介素性信号通路介导的炎症反应的优势。最后，对共表达基因及其网络进行了分析。结果揭示了恒河猴幼崽免疫系统对CA16反应的基因表达谱，并表明这种免疫反应是免疫系统积极动员的结果。这项初步的微阵列研究将为CA16感染的分子机制提供洞察力，并将有助于识别用于评估该病毒疫苗的生物标记物。(C)2016爱思唯尔B.V.保留所有权利。
Coxsackievirus A16 (CA16) is a dominant pathogen that results in hand, foot, and mouth disease and causes outbreaks worldwide, particularly in the Asia-Pacific region. However, the underlying molecular mechanisms remain unclear. Our previous study has demonstrated that the basic CA16 pathogenic process was successfully mimicked in rhesus monkey infant. The present study focused on the global gene expression changes in peripheral blood mononuclear cells of rhesus monkey infants with hand, foot, and mouth disease induced by CA16 infection at different time points. Genome-wide expression analysis was performed with Agilent whole-genome microarrays and established bioinformatics tools. Nine hundred and forty-eight significant differentially expressed genes that were associated with 5 gene ontology categories, including cell communication, cell cycle, immune system process, regulation of transcription and metabolic process were identified. Subsequently, the mapping of genes related to the immune system process by PANTHER pathway analysis revealed the predominance of inflammation mediated by chemokine and cytokine signaling pathways and the interleukin signaling pathway. Ultimately, co-expressed genes and their networks were analyzed. The results revealed the gene expression profile of the immune system in response to CA16 in rhesus monkey infants and suggested that such an immune response was generated as a result of the positive mobilization of the immune system. This initial microarray study will provide insights into the molecular mechanism of CA16 infection and will facilitate the identification of biomarkers for the evaluation of vaccines against this virus. (C) 2016 Elsevier B.V. All rights reserved.