Target profiling of zerumbone using a novel cell-permeable clickable probe and quantitative chemical proteomics
Target profiling of zerumbone using a novel cell-permeable clickable probe and quantitative chemical proteomics
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DOI：
10.1039/c4cc09527h
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发表时间：
2015-01-01
影响因子：
4.9
通讯作者：
Tate, Edward W.
中科院分区：
文献类型：
--
作者：
Kalesh, Karunakaran A.;Clulow, James A.;Tate, Edward W.
Zerumbone是一种植物化学物质，具有从抗炎到抗癌的多种生物活性;然而，迄今为止，这种重要化合物的细胞靶点仍然难以捉摸。在这里，我们报告的全球蛋白质靶谱zerumbone在活的癌细胞中使用基于竞争活性的蛋白质分析的一种新的细胞可渗透的点击探针，结合定量质谱。
Zerumbone is a phytochemical with diverse biological activities ranging from anti-inflammatory to anti-cancer properties; however, to date the cellular targets of this important compound have remained elusive. Here we report the global protein target spectrum of zerumbone in living cancer cells using competitive activity-based protein profiling of a novel cell-permeable clickable probe, combined with quantitative mass spectrometry.