Presence and formation of cobalamin analogues in multivitamin-mineral pills.
Presence and formation of cobalamin analogues in multivitamin-mineral pills.
复制标题
多种维生素矿物质丸中钴胺素类似物的存在和形成。
DOI：
10.1172/jci110685
复制
发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Allen,RH
中科院分区：
文献类型：
--
作者：
Kondo,H;Binder,MJ;Kolhouse,JF;Smythe,WR;Podell,ER;Allen,RH
由于钴胺素（维生素B12）类似物在动物饲料和动物和人类血液和组织中的来源是未知的，我们研究了多种维生素相互作用可能将钴胺素转化为钴胺素类似物的可能性。我们将三种流行的多种维生素矿物质药丸在水中均质化，在37 ℃下孵育2小时，并分离出钴胺素。使用纸色谱法，我们观察到20-90%的钴胺素以钴胺素类似物的形式存在。使用CN-[57 Co]钴胺素的研究表明，这些类似物是由于维生素C、硫胺素和铜对CN-钴胺素的协同作用而形成的。这些钴胺素类似物从小鼠的胃肠道吸收，并且当胃肠外注射时，不能刺激或实际上抑制钴胺素依赖性酶。我们得出结论，CN-钴胺素可以通过多种维生素-矿物质相互作用转化为潜在有害的钴胺素类似物，这些相互作用可能是导致动物饲料以及动物和人类血液和组织中存在钴胺素类似物的原因。
Because the origin of cobalamin (vitamin B12) analogues in animal chows and animal and human blood and tissues is unknown, we investigated the possibility that multivitamin interactions might convert cobalamin to cobalamin analogues. We homogenized three popular multivitamin-mineral pills in water, incubated them at 37 degrees C for 2 h, and isolated the cobalamin. Using paper chromatography we observed that 20-90% of the cobalamin was present as cobalamin analogues. Studies using CN-[57Co]cobalamin showed that these analogues were formed due to the concerted action of vitamin C, thiamine, and copper on CN-cobalamin. These cobalamin analogues are absorbed from the gastrointestinal tract of mice and either fail to stimulate or actually inhibit cobalamin-dependent enzymes when injected parenterally. We conclude that CN-cobalamin can be converted to potentially harmful cobalamin analogues by multivitamin-mineral interactions and that these interactions may be responsible for the presence of cobalamin analogues in animal chows and animal and human blood and tissues.