Activation of anthocyanin synthesis in Cymbidium orchids: variability between known regulators
Activation of anthocyanin synthesis in Cymbidium orchids: variability between known regulators
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DOI：
10.1007/s11240-009-9649-0
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发表时间：
2010-03-01
影响因子：
3
通讯作者：
Davies, Kevin M.
中科院分区：
文献类型：
--
作者：
Albert, Nick W.;Arathoon, Steve;Davies, Kevin M.
“Jung Frau dos Pueblos”（JDP）是一种商业性的白色兰花品种。在这项研究中，我们调查是否可以恢复花色素苷色素生产的JDP花瓣通过引入已知的花色素苷调节转录因子，通过基因枪转化。用玉米花色素苷调节剂Leaf color和Colorless 1同时转化白色JDP花瓣恢复了花色素苷生物合成，产生了猩红色花色素苷产生细胞的特征性生物射弹爆炸模式。不同的MYB/bHLH组合，以诱导花青素色素沉着在JDP花瓣的有效性进行了评估，我们证明，以前未表征的R2 R3 MYB转录因子AaMYB 1从红掌是能够激活花色素苷的生产在建兰花瓣。来自单子叶植物与双子叶植物物种的MYB因子诱导花青素色素沉着的有效性之间的明显区别表明单子叶植物和双子叶植物之间的转录因子的特异性，并且这可能由MYB因子决定。
Cymbidium hybrid 'Jung Frau dos Pueblos' (JDP) is a commercial white flowered orchid cultivar. In this study we investigated whether anthocyanin pigment production could be restored to JDP petals by introducing known anthocyanin-regulating transcription factors through biolistic transformation. Simultaneous transformation of white JDP petals with the maize anthocyanin regulators Leaf color and Colorless1 restored anthocyanin biosynthesis, producing a characteristic biolistic blast pattern of scarlet anthocyanin-producing cells. The effectiveness of different MYB/bHLH combinations to induce anthocyanin pigmentation in JDP petals was assessed, and we demonstrate that the previously uncharacterized R2R3 MYB transcription factor AaMYB1 from Anthurium andraeanum is capable of activating anthocyanin production in Cymbidium petals. The clear distinction between the effectiveness of MYB factors from monocot versus dicot species for inducing anthocyanin pigmentation suggests a specificity of transcription factors between monocots and dicots and that this may be determined by the MYB factor.