Exercise training delays renal disorders with decreasing oxidative stress and increasing production of 20-hydroxyeicosatetraenoic acid in Dahl salt-sensitive rats
Exercise training delays renal disorders with decreasing oxidative stress and increasing production of 20-hydroxyeicosatetraenoic acid in Dahl salt-sensitive rats
复制标题
DOI：
10.1097/hjh.0000000000002409
复制
发表时间：
2020-07-01
影响因子：
4.9
通讯作者：
Ito, Osamu
中科院分区：
文献类型：
--
作者：
Ogawa, Yoshiko;Takahashi, Junta;Ito, Osamu
目的：运动训练对人类和大鼠具有抗高血压和保护肾的作用。然而，运动训练对盐敏感性高血压肾病的影响尚不清楚。本研究旨在探讨运动训练对盐敏感性高血压大鼠肾脏功能的影响及其机制。方法：将6周龄雄性Dahl盐敏感大鼠分为正常盐（0.6% NaCl）饮食组、高盐（8% NaCl）饮食组和高盐饮食加运动训练组。高盐饮食加运动训练组每天在跑步机上跑步8周。结果：高盐饮食可引起严重高血压和肾功能不全。运动训练显著改善了高盐饮食引起的尿蛋白、白蛋白和l型脂肪酸结合蛋白的排泄，改善了肾小球硬化，但没有改变血压的肾间质纤维化。运动训练显著降低高盐饮食诱导的肾脏氧化应激，降低高盐饮食刺激的黄嘌呤氧化还原酶活性，但不降低烟酰胺腺嘌呤二核苷酸磷酸氧化酶活性。高盐饮食没有改变尿中20-羟基二糖四烯酸的排泄，也没有降低肾脏细胞色素P450 4A蛋白的表达。运动训练增加尿20-羟氧二碳四烯酸排泄和肾细胞色素P450 4A蛋白表达。结论：运动训练改善了Dahl盐敏感大鼠肾脏疾病，但没有降低血压。运动训练还能降低氧化应激，增加肾脏中20-羟基二糖四烯酸的产生。这些结果表明，改善氧化应激和20-羟基二碳四烯酸的产生可能是运动训练改善Dahl盐敏感大鼠肾脏疾病的潜在机制。
Objective: Exercise training has antihypertensive and renoprotective effects in humans and rats. However, the effects of exercise training on renal disorders that occur with salt-sensitive hypertension remains unclear. The study aim was to investigate the effects and mechanisms of exercise training on renal function in a rat model of salt-sensitive hypertension. Methods: Six-week-old male Dahl salt-sensitive rats were divided into normal-salt (0.6% NaCl) diet, high-salt (8% NaCl) diet, and high-salt diet with exercise training groups. The high-salt diet with exercise training group underwent daily treadmill running for 8 weeks. Results: The high-salt diet induced severe hypertension and renal dysfunction. Exercise training significantly improved high-salt diet-induced urinary protein, albumin, andl-type fatty acid-binding protein excretion, and glomerulosclerosis but not renal interstitial fibrosis without changing blood pressure. Exercise training significantly attenuated high-salt diet-induced oxidative stress in the kidneys and decreased high-salt diet-stimulated xanthine oxidoreductase activity but not nicotinamide adenine dinucleotide phosphate oxidase activity. The high-salt diet did not change urinary excretion of 20-hydroxyeicosatetraenoic acid and decreased cytochrome P450 4A protein expression in the kidneys. Exercise training increased urinary 20-hydoroxyeicosatetraenoic acid excretion and renal cytochrome P450 4A protein expression. Conclusion: Exercise training improved renal disorders without lowering blood pressure in Dahl salt-sensitive rats. Exercise training also decreased oxidative stress and increased 20-hydroxyeicosatetraenoic acid production in the kidneys. These results suggest that improvements in oxidative stress and 20-hydroxyeicosatetraenoic acid production may be potential mechanisms by which exercise training improved renal disorders in Dahl salt-sensitive rats.