Differential regulation of HLA‐DR mRNAs and cell surface antigens by interferon.
Differential regulation of HLA‐DR mRNAs and cell surface antigens by interferon.
复制标题
干扰素对 HLA-DR mRNA 和细胞表面抗原的差异调节。
作者：
F. Rosa;D. Hatat;A. Abadie;D. Wallach;M. Revel;M. Fellous
人类干扰素- α、- β和- γ在所有研究的人类淋巴母细胞样细胞和黑色素瘤细胞系中增强HLA - DR mrna。这种增加涉及α链和β链mrna。此外，我们发现免疫干扰素- γ优先增强II类MHC mRNA。IFN - γ对α和β HLA - DR链合成的影响也通过免疫沉淀法进行了分析。它被一种针对人IFN - γ的单克隆抗体所消除。干扰素对细胞表面HLA - DR分子水平的影响并不总是与HLA - DR mRNA的增强相对应。我们的实验表明，这种HLA - DR mRNA和细胞表面抗原增强之间的差异可能是由于所研究的几种人类细胞上相应抗原的组成性高水平所致。
Human interferons‐alpha, ‐beta and ‐gamma enhance HLA‐DR mRNAs in all the human lymphoblastoid and melanoma cell lines studied. The increase concerns both alpha and beta chain mRNAs. Moreover, we show that immune interferon‐gamma preferentially enhances class II MHC mRNA. This effect of IFN‐gamma on the synthesis of alpha and beta HLA‐DR chains has been also analysed by immunoprecipitation. It is abolished by a monoclonal antibody directed against human IFN‐gamma. The effect of interferon on the cell surface level of HLA‐DR molecules does not always correspond to the enhancement of HLA‐DR mRNA. Our experiments suggest that this discrepancy between the enhancement of HLA‐DR mRNA and cell surface antigen might be due to a constitutively high level of the corresponding antigens on several of the human cells studied.