Advancing animal models of neoplasia through in vivo bioluminescence imaging
Advancing animal models of neoplasia through in vivo bioluminescence imaging
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DOI：
10.1016/s0959-8049(02)00410-0
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发表时间：
2002-11-01
影响因子：
8.4
通讯作者：
Contag, CH
中科院分区：
文献类型：
--
作者：
Edinger, M;Cao, YA;Contag, CH
恶性疾病是复杂的分子和细胞事件的最终表现，导致不受控制的细胞增殖并最终导致组织破坏和转移。虽然在体外培养的肿瘤细胞的检查允许在细胞和亚细胞水平上的肿瘤发生的早期途径的分子解剖，只有在活体动物的器官系统的复杂性内的这些过程的询问可以揭示肿瘤性疾病中发生的全部病理生理变化。这种分析需要促进体内生物过程研究的技术，在过去几年中已经开发了几种方法。在体内分子和细胞成像的新生领域中，这些策略将联合收割机分子生物学与成像方式结合，作为实时获取关于生命系统中疾病过程的功能信息的手段。在这篇综述中，我们将总结在体内生物发光成像（BLI）的最新进展，并讨论这种成像策略的癌症研究的未来的潜力。(C)2002爱思唯尔科技有限公司版权所有。
Malignant disease is the final manifestation of complex molecular and cellular events leading to uncontrolled cellular proliferation and eventually tissue destruction and metastases. While the in vitro examination of cultured tumour cells permits the molecular dissection of early pathways in tumorigenesis on cellular and subcellular levels, only interrogation of these processes within the complexity of organ systems of the living animal can reveal the full range of pathophysiological changes that occur in neoplastic disease. Such analyses require technologies that facilitate the study of biological processes in vivo, and several approaches have been developed over the last few years. These strategies, in the nascent field of in vivo molecular and cellular imaging, combine molecular biology with imaging modalities as a means to real-time acquisition of functional information about disease processes in living systems. In this review, we will summarise recent developments in in vivo bioluminescence imaging (BLI) and discuss the potential of this imaging strategy for the future of cancer research. (C) 2002 Elsevier Science Ltd. All rights reserved.