High-resolution histographical mapping of glucose concentrations in developing cotyledons of Vicia faba in relation to mitotic activity and storage processes:: glucose as a possible developmental trigger
High-resolution histographical mapping of glucose concentrations in developing cotyledons of Vicia faba in relation to mitotic activity and storage processes:: glucose as a possible developmental trigger
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DOI：
10.1046/j.1365-313x.1998.00214.x
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发表时间：
1998-08-01
期刊：
影响因子：
7.2
通讯作者：
Wobus, U
中科院分区：
文献类型：
--
作者：
Borisjuk, L;Walenta, S;Wobus, U
以前的研究提供了证据，证明碳水化合物状态会触发蚕豆生长过程中的子叶发育过程。在这里，我们描述了利用定量生物发光和单光子成像技术对蚕豆子叶发育过程中组织切片中葡萄糖浓度的高分辨率定位。将局部葡萄糖分布模式与组织细胞类型、有丝分裂指数和淀粉分布模式进行比较。在子叶分化过程中，葡萄糖浓度出现梯度，这与特定的细胞类型有关。在子叶的未分化的早熟区域发现较高的浓度，而成熟的淀粉积累区域含有特别低浓度的葡萄糖。此外，葡萄糖浓度与有丝分裂活性有关。因此，葡萄糖的分布模式与发育梯度有关。我们的数据首次提供了植物胚胎发育过程中葡萄糖梯度陡峭的证据，并支持糖梯度可能在子叶发育中具有形态发生功能的观点。
Previous studies provided evidence that the carbohydrate status triggers developmental processes in the growing cotyledons of Vicia faba. We describe here the high-resolution mapping of glucose concentrations in tissue sections of developing faba bean cotyledons by quantitative bioluminescence and single-photon imaging. Patterns of local glucose distributions are compared with tissue cell type, mitotic index and the distribution pattern of starch. During cotyledon differentiation, gradients in the glucose concentration emerge which are related to the particular cell type. Higher concentrations are found in non-differentiated premature regions of the cotyledon whereas mature starch-accumulating regions contain particularly low concentrations of glucose. In addition, glucose concentration is correlated to mitotic activity. The glucose distribution pattern is therefore related to the developmental gradient. Our data provide for the first time evidence for steep glucose gradients across developing plant embryos and favour the idea that sugar gradients may have morphogenic functions in developing cotyledons.