Time-of-Day-Dependent Variations of Scratching Behavior and Transepidermal Water Loss in Mice that Developed Atopic Dermatitis
Time-of-Day-Dependent Variations of Scratching Behavior and Transepidermal Water Loss in Mice that Developed Atopic Dermatitis
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DOI：
10.1292/jvms.14-0173
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发表时间：
2014-11-01
影响因子：
1.2
通讯作者：
Oku, Keisuke
中科院分区：
文献类型：
--
作者：
Ohmori, Keitaro;Minamide, Kana;Oku, Keisuke
痉挛和皮肤屏障功能障碍是特应性皮炎（AD）的关键特征和治疗靶点;然而，这些特征的时间依赖性变化仍不清楚。NC/Tnd小鼠在空气不受控制的环境下饲养时，表现出严重的抓挠行为和皮肤屏障破坏，以及自发性特应性皮炎的发展。在本研究中，在发展为中度至重度AD的NC/Tnd小鼠中评价了抓挠行为和经表皮水分丢失（TEWL）的时间依赖性变化。对小鼠24小时的分析表明，从下午到夜间，当自发活动较低时，抓挠频率和持续时间增加，而抓挠行为在上午减少。最高抓挠频率和持续时间分别是最低抓挠频率和持续时间的3.8倍和4.1倍。此外，背侧皮损的TEWL在白天减少，而在夜间增加。最高TEWL是最低TEWL的1.3倍。在抓挠频率和持续时间以及TEWL中检测到显著的每日变化。这些结果表明，NC/Tnd小鼠是研究与AD相关的抓挠行为和皮肤屏障功能障碍的时间依赖性变化的合适小鼠模型。
Scratching and skin barrier dysfunctions are pivotal features and therapeutic targets of atopic dermatitis (AD); however, time-of-day-dependent variations of these characteristics remain unclear. NC/Tnd mice have been shown to exhibit severe scratching behavior and skin barrier disruption together with the development of spontaneous atopic dermatitis when they are raised under air-uncontrolled environment. In the present study, time-of-day-dependent variations of scratching behavior and transepidermal water loss (TEWL) were evaluated in NC/Tnd mice that developed moderate to severe AD. Analysis of the mice for 24 hr revealed that scratching frequency and duration were increased from in the afternoon to the nocturnal period when locomotor activity was low, and scratching behavior was decreased in the morning. The highest scratching frequency and duration were 3.8- and 4.1-fold increases in the lowest scratching frequency and duration, respectively. In addition, TEWL on the dorsal skin lesion was decreased in the diurnal period, while that was increased in the nocturnal period. The highest TEWL was a 1.3-fold increase in the lowest TEWL. Significant daily variations were detected in scratching frequency and duration and TEWL. These results indicate that NC/Tnd mice are an appropriate mouse model to investigate time-of-day-dependent variations of scratching behavior and skin barrier dysfunctions associated with AD.