Factors influencing real time internal structural visualization and dynamic process monitoring in plants using synchrotron-based phase contrast X-ray imaging.
Factors influencing real time internal structural visualization and dynamic process monitoring in plants using synchrotron-based phase contrast X-ray imaging.
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DOI：
10.1038/srep12119
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发表时间：
2015-07-17
影响因子：
4.6
通讯作者：
Hallin E
中科院分区：
文献类型：
--
作者：
Karunakaran C;Lahlali R;Zhu N;Webb AM;Schmidt M;Fransishyn K;Belev G;Wysokinski T;Olson J;Cooper DM;Hallin E
对植物部分（根、茎、叶和花）的微创调查具有很好的潜力来阐明植物生长、形态、生理和根-根际相互作用的动态。基于实验室的吸收X射线成像和计算机断层扫描（CT）系统被广泛用于在自然和人造土壤中生长的植物的原位可行性研究。这些技术具有诸如土壤孔隙空间和根之间的低对比度、长X射线成像时间和低空间分辨率的挑战。在这项研究中，使用同步加速器（SR）的相位衬度X射线成像（PCI）已被证明是一种微创的植物成像技术。对生长在桑迪粘壤土上的10日龄油菜和小麦幼苗的地上部分和根系进行了成功的扫描和重建。结果表明，SR-PCI可以提供高质量的图像，研究动态和真实的时间过程，如空化和水再填充在植物。阐述了SR-PCI技术的优势、X射线能量效应和有效像素尺寸对植物样品研究的影响。本文还对造影剂在植物生理过程监测中的应用进行了研究和讨论。
Minimally invasive investigation of plant parts (root, stem, leaves, and flower) has good potential to elucidate the dynamics of plant growth, morphology, physiology, and root-rhizosphere interactions. Laboratory based absorption X-ray imaging and computed tomography (CT) systems are extensively used for in situ feasibility studies of plants grown in natural and artificial soil. These techniques have challenges such as low contrast between soil pore space and roots, long X-ray imaging time, and low spatial resolution. In this study, the use of synchrotron (SR) based phase contrast X-ray imaging (PCI) has been demonstrated as a minimally invasive technique for imaging plants. Above ground plant parts and roots of 10 day old canola and wheat seedlings grown in sandy clay loam soil were successfully scanned and reconstructed. Results confirmed that SR-PCI can deliver good quality images to study dynamic and real time processes such as cavitation and water-refilling in plants. The advantages of SR-PCI, effect of X-ray energy, and effective pixel size to study plant samples have been demonstrated. The use of contrast agents to monitor physiological processes in plants was also investigated and discussed.