Enzymatic hydrolysis at high-solids loadings for the conversion of agave bagasse to fuel ethanol
Enzymatic hydrolysis at high-solids loadings for the conversion of agave bagasse to fuel ethanol
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DOI：
10.1016/j.apenergy.2013.07.036
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发表时间：
2014-01-01
期刊：
影响因子：
11.2
通讯作者：
Martinez, Alfredo
中科院分区：
文献类型：
--
作者：
Caspeta, Luis;Caro-Bermudez, Mario A.;Martinez, Alfredo
龙舌兰甘蔗渣是墨西哥酒精饮料生产过程中积累的木质纤维素残留物，是生产生物燃料的潜在原料。采用析因设计研究了温度、停留时间、酸浓度和乙醇浓度对乙醇溶剂预处理和酶解龙舌兰渣的影响。该方法和使用搅拌的自制微型反应器将龙舌兰甘蔗渣的消化率从用稀酸法观察到的30%增加到98%;还允许减少用于水解样品的酶的量，其中固体负载为30%w/w，并且在酶水解产物中获得高达225 g/L的葡萄糖浓度。总的来说，该方法允许回收包含在龙舌兰甘蔗渣中的总可发酵糖的91%（0.51g/g）和作为副产物的总木质素的69%（0.11g/g）。在最佳条件下，使用工业酵母菌株发酵的最大乙醇产率为0.25 g/g干龙舌兰甘蔗渣，这是最大理论值（0.29 g/g）的86%。的葡萄糖浓度和固体负载的纤维素转化为葡萄糖的效果进行了讨论，除了预期的生产约50万升的燃料乙醇使用龙舌兰酒工业的龙舌兰甘蔗渣残留物作为一个潜在的解决方案的处置问题。(C)2013爱思唯尔有限公司保留所有权利。
Agave bagasse is the lignocellulosic residue accumulated during the production of alcoholic beverages in Mexico and is a potential feedstock for the production of biofuels. A factorial design was used to investigate the effect of temperature, residence time and concentrations of acid and ethanol on ethanosolv pretreatment and enzymatic hydrolysis of agave bagasse. This method and the use of a stirred in-house-made mini-reactor increased the digestibility of agave bagasse from 30% observed with the dilute-acid method to 98%; also allowed reducing the quantity of enzymes used to hydrolyze samples with solid loadings of 30% w/w and glucose concentrations up to 225 g/L were obtained in the enzymatic hydrolysates. Overall this process allows the recovery of 91% of the total fermentable sugars contained in the agave bagasse (0.51 g/g) and 69% of total lignin as co-product (0.11 g/g). The maximum ethanol yield under optimal conditions using an industrial yeast strain for the fermentation was 0.25 g/g of dry agave bagasse, which is 86% of the maximum theoretical (0.29 g/g). The effect of the glucose concentration and solid loading on the conversion of cellulose to glucose is discussed, in addition to prospective production of about 50 million liters of fuel ethanol using agave bagasse residues from the tequila industry as a potential solution to the disposal problems. (C) 2013 Elsevier Ltd. All rights reserved.