Decreased expression of carbonic anhydrase isozyme II, rather than of isozyme VI, in submandibular glands in long-term zinc-deficient rats
Decreased expression of carbonic anhydrase isozyme II, rather than of isozyme VI, in submandibular glands in long-term zinc-deficient rats
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DOI：
10.1017/s0007114507801565
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发表时间：
2008-02-01
影响因子：
3.6
通讯作者：
Komai, Michio
中科院分区：
文献类型：
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作者：
Goto, Tomoko;Shirakawa, Hitoshi;Komai, Michio
我们以前报道，在大鼠中，长期缺锌显着降低味觉敏感性和总碳酸酐酶（CA）的活性在颌下腺。因此，我们研究了锌缺乏对唾液分泌和CA同工酶（II和VI）在大鼠颌下腺的表达的影响，因为这些同工酶被认为是与味觉和唾液分泌。雄性Sprague-Dawley大鼠，4周龄。试验分为3组，分别饲喂含锌2.2、4.1和33.7mg/kg的日粮，试验期42 d。北方印迹分析表明，锌缺乏降低CA II mRNA的表达在下颌下腺，而不降低CA VI mRNA的表达。在蛋白质印迹分析中，锌缺乏显着降低CA II（红细胞CA）蛋白在颌下腺的表达，而不降低CA VI蛋白的表达。唾液分泌低于锌-def组比在配对喂养组。这些结果表明，降低CA同工酶II的表达的基础上降低CA活性先前报道在Zn-def大鼠的下颌下腺，这可能会减少定期唾液分泌。
We previously reported that in rats, long-term Zn deficiency significantly reduced taste sensitivity and total carbonic anhydrase (CA) activity in the submandibular gland. We therefore investigated the effects of Zn deficiency on salivary secretion and the expressions of CA isozymes (II and VI) in the rat submandibular gland, since those isozymes are thought to be related to taste sensation and salivary secretion. Male Sprague-Dawley rats, age 4 weeks. were divided into three groups (Zn-def, low-Zn and pair-fed, that were fed a diet containing 2.2, 4.1 or 33.7 mg Zn/kg, respectively, for 42 d). Northern, blot analysis indicated that Zn deficiency reduced CA II mRNA expression in the submandibular gland without reducing CA VI mRNA expression. In Western blot analysis, Zn deficiency significantly reduced CA II (erythrocyte CA) protein expression in the submandibular gland without reducing CA VI protein expression. Salivary secretion was lower in the Zn-def group than in the pair-fed group. These results suggest that decreased CA isozyme II expression underlies the decreased CA activity previously reported in the submandibular gland in Zn-def rats, and this may reduce regular salivary secretion.