Characterization of a new human diploid myeloid leukemia cell line (PLB-985) with granulocytic and monocytic differentiating capacity.
Characterization of a new human diploid myeloid leukemia cell line (PLB-985) with granulocytic and monocytic differentiating capacity.
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DOI：
10.1182/blood.v70.2.372.bloodjournal702372
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发表时间：
1987-08
期刊：
影响因子：
20.3
通讯作者：
K. A. Tucker;M. Lilly;L. Heck;T. Rado
中科院分区：
文献类型：
--
作者：
K. A. Tucker;M. Lilly;L. Heck;T. Rado
从急性非淋巴细胞白血病（ANLL）患者外周血中建立了一个新的人二倍体细胞系，命名为PLB-985。该细胞系的细胞能够在诱导剂的存在下粒细胞和单核细胞成熟。经形态学、表面抗原分析和细胞化学染色证实PLB-985细胞为髓单核母细胞。透射电子显微镜显示，它们是缺乏嗜酸性初级或次级颗粒，并有一个开放的染色质模式与频繁的核仁。该品系的典型核型为46，XX，没有一致的标记染色体或可识别的易位。该细胞系的成髓细胞在软琼脂中形成集落，并在裸鼠中诱导肿瘤（绿色瘤）。在二甲亚砜、顺式视黄酸或二丁酰环磷酸腺苷存在下，细胞生长导致粒细胞成熟，这通过形态学、组织化学染色特征以及35 S-甲硫氨酸掺入中性粒细胞初级颗粒蛋白酶弹性蛋白酶和组织蛋白酶G来确定。促进肿瘤的佛波醇酯佛波醇肉豆蔻酸酯乙酸酯诱导PLB-985细胞分化为单核细胞。在该试剂存在下生长的细胞迅速粘附到塑料上，显示出胶乳颗粒的吞噬作用显著增加，α-乙酸萘酯酶染色阳性，并失去合成亲脂蛋白酶的能力。分化的诱导沿着任一途径都伴随着myc癌基因转录的显著降低。
A new human diploid cell line, designated PLB-985, has been established from the peripheral blood of a patient with acute nonlymphocytic leukemia (ANLL). Cells of this line are capable of granulocytic and monocytic maturation in the presence of inducing agents. By morphology, the analysis of surface antigens, and cytochemical staining PLB-985 cells are myelomonoblasts. Transmission electron microscopy reveals them to be devoid of neutrophilic primary or secondary granules and to have an open chromatin pattern with frequent nucleoli. The modal karyotype of the line is 46,XX, with no consistent marker chromosomes or recognizable translocations. Myelomonoblasts of this line form colonies in soft agar and induce tumors (chloromas) in nude mice. Growth of the cells in the presence of dimethyl sulfoxide, cis-retinoic acid, or dibutyryl cyclic adenosine monophosphate results in granulocytic maturation as determined by morphology, histochemical staining characteristics, and incorporation of 35S-methionine into the neutrophil primary granule proteinases elastase and cathepsin G. The tumor-promoting phorbol ester phorbol myristate acetate induces PLB-985 cells to differentiate as monocytes. Cells grown in the presence of this agent rapidly become adherent to plastic, display markedly increased phagocytosis of latex particles, stain positively for alpha-naphthyl acetate esterase, and lose the ability to synthesize the neutrophilic proteinases. Induction of differentiation along either pathway is accompanied by a marked decrease in myc oncogene transcription.