Overexpression of RGC-32 in colon cancer and other tumors
Overexpression of RGC-32 in colon cancer and other tumors
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DOI：
10.1016/j.yexmp.2004.11.001
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发表时间：
2005-04-01
影响因子：
3.6
通讯作者：
Rus, H
中科院分区：
文献类型：
--
作者：
Fosbrink, M;Cudrici, C;Rus, H
肿瘤通常表现出细胞周期的失调和细胞周期蛋白和细胞周期蛋白依赖性激酶（CDK）的过表达。补体应答基因（RGC）-32是CDC 2的底物和调节因子，其过表达可诱导细胞周期激活。通过使用表达阵列技术，我们发现19%的肿瘤组织显示RGC-32 mRNA表达高于相应的正常组织水平，另一方面，在70%的结肠腺癌样品中发现RGC-32蛋白表达增加。在结肠癌中，观察到两种主要的RGC-32免疫反应性模式：仅在某些肿瘤中恶性上皮细胞染色，而在其他肿瘤中恶性上皮细胞和结肠癌细胞的RGC-32反应性。从正常个体获得的结肠上皮对RGC-32蛋白始终呈阴性。RGC-32蛋白在前列腺、膀胱、乳腺等肿瘤中也有过表达。肺和其他消化道肿瘤。RGC-32表达存在于同样表达增殖标志物Ki-67的恶性上皮细胞中。我们的数据表明，RGC-32过表达可能是肿瘤细胞生长所需的细胞周期失调的一部分。All rights reserved.
Tumors often exhibit deregulation of the cell cycle and overexpression of cyclins and cyclin-dependent kinases (CDKs). Response gene to complement (RGC)-32 is a substrate and regulator of CDC2 and its overexpression induces cell cycle activation, We investigated RGC-32 mRNA and protein expression in tumors with special emphasis in colon carcinoma. By using an expression array technique we found that 19% of tumor tissues showed increased RGC-32 mRNA expression over the levels of corresponding normal tissues, On the other hand, an increased RGC-32 protein was found in 70% of colon adenocarcinoma samples tested. In colon carcinomas, two major patterns of RGC-32 immunoreactivity were seen: staining of malignant epithelial cells only in some tumors and RGC-32 reactivity of both malignant epithelia as well as cells in the interstitium in others. Colonic epithelium obtained from normal individuals was consistently negative for RGC-32 protein. Overexpression of RGC-32 protein was found in other tumors including prostate, bladder, breast. lung, and other digestive tract tumors. RGC-32 expression was present in the same malignant epithelial cells that also expressed the proliferation marker Ki-67. Our data suggest that RGC-32 overexpression might be part of the deregulation of the cell cycle that is required for the growth of tumor cells, (c) 2004 Elsevier Inc. All rights reserved.