RPLC-Ion-Trap-FTMS Method for Lipid Profiling of Plasma: Method Validation and Application to p53 Mutant Mouse Model
RPLC-Ion-Trap-FTMS Method for Lipid Profiling of Plasma: Method Validation and Application to p53 Mutant Mouse Model
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DOI：
10.1021/pr800373m
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发表时间：
2008-11-01
影响因子：
4.4
通讯作者：
Hankemeier, Thomas
中科院分区：
文献类型：
--
作者：
Hu, Chunxiu;van Dommelen, Judith;Hankemeier, Thomas
建立了反相液相色谱-线性离子陷阱-傅立叶变换离子回旋共振-质谱法测定人和小鼠血浆中脂质的方法。使用融合核心C-8柱和二元梯度，在一次LC-MS运行中检测到属于8个不同类别的160多种脂类。该方法具有良好的线性关系(R2，0.994~1.000)、检出限(0.08~1.28mUg/mL血浆)、重现性(RSD2.7~7.9%)和中等精密度(RSD2.7~15.6%)。将该方法成功地应用于p53基因突变小鼠血浆中，研究了p53基因表达的某些表型效应。
A reversed-phase liquid chromatography-linear ion trap-Fourier transform ion cyclotron resonance-mass spectrometric method was developed for the profiling of lipids in human and mouse plasma. With the use of a fused-core C-8 column and a binary gradient, more than 160 lipids belonging to eight different classes were detected in a single LC-MS run. The method was fully validated and the analytical characteristics such as linearity (R-2, 0.994-1.000), limit of detection (0.08-1.28 mu g/mL plasma), repeatability (RSD, 2.7-7.9%) and intermediate precision (RSD, 2.7-15.6%) were satisfactory. The method was successfully applied to p53 mutant mice plasma for studying some phenotypic effects of p53 expression.