An Active Fraction of Achyranthes bidentata Polypeptides Prevents Apoptosis Induced by Serum Deprivation in SH-SY5Y Cells Through Activation of PI3K/Akt/Gsk3β Pathways
An Active Fraction of Achyranthes bidentata Polypeptides Prevents Apoptosis Induced by Serum Deprivation in SH-SY5Y Cells Through Activation of PI3K/Akt/Gsk3β Pathways
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DOI：
10.1007/s11064-011-0543-x
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发表时间：
2011-11-01
影响因子：
4.4
通讯作者：
Ding, Fei
中科院分区：
文献类型：
--
作者：
Shen, Yuntian;Zhang, Qi;Ding, Fei
牛膝是一种常用的中草药。我们以前的研究已经证明了牛膝多肽(ABPP)的神经保护作用，ABPP是牛膝水提物中的主要成分。现在，我们已经用高效液相色谱法从ABPP中分离出一种活性成分，称为ABPP-E4。本研究旨在探讨ABPP-E4可能的治疗潜力。细胞存活率和细胞凋亡率检测表明，ABPP-E4以浓度依赖的方式拮抗无血清培养的SH-SY5Y细胞的存活率下降和细胞凋亡。ABPP-E4预处理还导致血清剥夺条件下细胞内Bcl2/Bax比值升高，caspase-3活性降低。信号通路分析表明，ABPP-E4可刺激培养的SH-SY5Y细胞Akt/GSK3β信号的激活，其抗凋亡作用可被PI3K的化学抑制所阻断。综上所述，ABPP-E4可能通过调节PI3K/Akt/GSK3β通路对血清剥夺诱导的神经细胞凋亡起到保护作用。
Achyranthes bidentata Blume is a commonly prescribed Chinese medicinal herb. Our previous studies have proved the neuroprotective function of Achyranthes bidentata polypeptides (ABPP), a major constituent from aqueous extracts of the herb. Now we have separated an active fraction, referred to as ABPP-E4, from ABPP by HPLC methods. This study aimed to investigate the possible therapeutic potential of ABPP-E4. Assessments of cell viability and apoptosis indicated that ABPP-E4 pretreatment, in a concentration-dependent manner, antagonized the cell viability loss and cell apoptosis of cultured SH-SY5Y cells deprived of serum. ABPP-E4 pretreatment also resulted in increase of Bcl-2/Bax ratio and inhibition of caspase-3 activation in the cells on exposure to serum deprivation. Signaling pathway analysis indicated that ABPP-E4 treatment stimulated the activation of Akt/Gsk3 beta signaling in cultured SH-SY5Y cells, and anti-apoptotic effects of ABPP-E4 could be blocked by chemical inhibition of PI3K. Taken together, all the results suggest that ABPP-E4 might exert protective effects against serum deprivation-induced neuronal apoptosis through modulation of PI3K/Akt/Gsk3 beta pathways.