FOS-like antigen 1 is highly expressed in human psoriasis tissues and promotes the growth of HaCaT cells in vitro.
FOS-like antigen 1 is highly expressed in human psoriasis tissues and promotes the growth of HaCaT cells in vitro.
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DOI：
10.3892/mmr.2014.2509
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发表时间：
2014-11
影响因子：
3.4
通讯作者：
Wu Zhu;Jing Li;Juan Su;Jie Li;Jinmao Li;Bo Deng;Qian Shi;Yanhong Zhou;Xiang Chen
中科院分区：
文献类型：
--
作者：
Wu Zhu;Jing Li;Juan Su;Jie Li;Jinmao Li;Bo Deng;Qian Shi;Yanhong Zhou;Xiang Chen
银屑病是一种多因素疾病，其发病机制仍有待阐明。 FOS 样抗原 1 (Fra-1) 是一种原癌基因。它是激活蛋白-1 活性的负抑制剂并具有转化活性。 Fra-1 在银屑病中的作用和可能的机制仍有待阐明。在本研究中，使用蛋白质印迹分析和逆转录定量聚合酶链反应 (RT-qPCR) 技术来鉴定银屑病组织和正常对照组织中差异表达的 Fra-1。与对照样本相比，银屑病中标准化 Fra-1 基因的表达量高出 12.6 倍。 Western blot 分析用于评估 Fra-1 的蛋白水平。结果表明，Fra-1 蛋白在银屑病影响的组织中表达较高。这也与 RT-qPCR 的结果相符。然后生成 Fra-1 稳定表达 HaCaT/Fra-1 或对照 HaCaT/载体细胞系，以阐明 Fra-1 在 HaCaT 细胞生长中的功能。结果表明，Fra-1 通过阻滞细胞周期和抑制细胞凋亡来促进 HaCaT 细胞的体外生长。这些结果表明 Fra-1 在银屑病中可能很重要。
Psoriasis is a multifactorial disease and the mechanisms involved in its pathogenesis remain to be elucidated. FOS‑like antigen 1 (Fra‑1) is a proto‑oncogene. It is a negative inhibitor of activator protein‑1 activity and possesses transforming activity. The effect of and possible mechanisms underlying Fra‑1 in psoriasis remain to be elucidated. In the present study, western blot analysis and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) techniques were used to identify differentially expressed Fra‑1 in psoriatic and in normal control tissues. Compared with the control samples, the expression of normalized Fra‑1 genes in psoriasis was 12.6 times higher. Western blot analysis was used to assess the protein levels of Fra‑1. The results demonstrated that the protein expression of Fra-1 was high in tissues affected by psoriasis. This also corresponded with the results of RT‑qPCR. Fra‑1‑stable expressing HaCaT/Fra‑1 or control HaCaT/vector cell lines were then generated to elucidate the function of Fra‑1 in the growth of HaCaT cells. The results demonstrated that Fra‑1 promoted the growth of HaCaT cells in vitro by arresting the cell cycle and inhibiting cell apoptosis. These results suggested that Fra‑1 may be important in psoriasis.