Human pancreatic adenocarcinoma: In vitro and in vivo morphology of a new tumor line established from ascites
Human pancreatic adenocarcinoma: In vitro and in vivo morphology of a new tumor line established from ascites
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DOI：
10.1007/bf02796382
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
W. H. Chen;J. Horoszewicz;S. Leong;T. Shimano;R. Penetrante;W. H. Sanders;R. Berjian;H. Douglass
中科院分区：
文献类型：
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作者：
W. H. Chen;J. Horoszewicz;S. Leong;T. Shimano;R. Penetrante;W. H. Sanders;R. Berjian;H. Douglass
已经从患有组织病理学证实的胰头腺癌的患者的腹水建立了人胰腺肿瘤细胞系，并在实验室中维持超过12个月。上皮样肿瘤细胞集落，这是由于原代组织培养的腹水细胞成分，机械分离针显微操作。在无胸腺裸鼠中证实了致瘤性。形态学上，胰腺肿瘤上皮细胞生长至汇合，与培养塑料表面适度紧密粘附，并在培养基中自由漂浮细胞。在组织培养中重新建立肿瘤异种移植物后，上皮细胞保持其原始形态。在裸鼠中生长的肿瘤在组织学上表现出患者中原发性腺癌的原型特征，产生丰富的粘蛋白并显示出广谱的腺分化，其范围从分化良好到分化不良的腺癌，偶尔局部淋巴细胞浸润。此外，肿瘤表达癌胚抗原和人胰腺癌相关抗原。该肿瘤系命名为AsPC-1，已在体外培养至少10代，在体内培养至少3代。它代表了人类胰腺癌的新模型。
A human pancreatic tumor cell line has been established from the ascites of a patient with histopathologically confirmed adenocarcinoma of the head of the pancreas and maintained for more than 12 months in the laboratory. Epitheloid tumor cell colonies, which resulted from primary tissue cultures of the ascitic cell component, were mechanically isolated by needle micromanipulation. Tumorigenicity was proven in athymic nude mice. Morphologically the pancreatic tumor epithelial cells grew to confluency with moderately tight adhesion to the culture plastic surface and with free-floating cells in the medium. Upon re-establishment of the tumoral xenograft in tissue culture, the epithelial cells retained their original morphology. Histologically the tumor grown in nude mice exhibited prototypic characteristics of the primary adenocarcinoma in the patient, producing abundant mucin and displaying a broad spectrum of glandular differentiation, which ranged from well to poorly differentiated adenocarcinomas with occasionally localized lymphocytic infiltrations. Furthermore, the tumor expressed carcinoembryonic antigen and human pancreas cancer associated antigen. This tumor line, designated AsPC-1, has been cultured for at least 10 passages in vitro and 3 in vivo. It represents a new model for human pancreatic cancer.