Population genetic structure of the thick-tailed bushbaby ( Otolemur crassicaudatus ) from the Soutpansberg Mountain range, Northern South Africa, based on four mitochondrial DNA regions
Population genetic structure of the thick-tailed bushbaby ( Otolemur crassicaudatus ) from the Soutpansberg Mountain range, Northern South Africa, based on four mitochondrial DNA regions
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基于四个线粒体 DNA 区域的来自南非北部 Soutpansberg 山脉的粗尾丛林宝宝 (Otolemur crassicaudatus) 的种群遗传结构
DOI：
10.1080/24701394.2019.1694015
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发表时间：
2020
影响因子：
--
通讯作者：
Kotze, Antoinette
中科院分区：
文献类型：
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作者：
Phukuntsi, Metlholo Andries;Du Plessis, Morne;Dalton, Desiré Lee;Jansen, Raymond;Cuozzo, Frank P.;Sauther, Michelle L.;Kotze, Antoinette
大猩猩，链鼻灵长类动物，分布在非洲中部，东部和南部，北方和南非东部代表了该物种最南部的分布。大熊猫是栖息地专家，其自然分散的栖息地由于人类活动而变得更加分散。目前，没有关于该物种的种群遗传数据或研究发表。本研究的目的是调查南非林波波省Soutpansberg山脉的粗尾耳鼻喉科幼鱼（Otolemur crassicaudatus）的遗传变异。四个线粒体区域，从高度保守到高度可变，从47个个体进行测序。对序列进行比对，并进行遗传多样性、结构和人口统计学分析。低的遗传多样性（编码区π = 0.0007-0.0038，非编码区π = 0.0127;编码区Hd = 0.166-0.569，非编码区Hd = 0.584）和亚结构（编码区H= 2-3，非编码区H = 4），有两个不同的单倍型群（编码区单倍型成对距离= 3-5，非编码区单倍型成对距离= 6-10）。这表明，人口可能经历了固定的线粒体单倍型，由于有限的女性移民，这是符合philopatric物种，替代单倍型不是本地的这个人口，可能有男性流动性从相邻的人口。这项研究提供了第一个详细的见解的线粒体遗传多样性的大陆非洲链鼻灵长类动物，并证明了线粒体DNA的效用，这些灵长类动物的种内遗传种群分析。
Greater bushbabies, strepsirrhine primates, that are distributed across central, eastern and southern Africa, with northern and eastern South Africa representing the species’ most southerly distribution. Greater bushbabies are habitat specialists whose naturally fragmented habitats are getting even more fragmented due to anthropogenic activities. Currently, there is no population genetic data or study published on the species. The aim of our study was to investigate the genetic variation in a thick-tailed bushbaby,Otolemur crassicaudatus,population in the Soutpansberg mountain range, Limpopo Province, South Africa. Four mitochondrial regions, ranging from highly conserved to highly variable, were sequenced from 47 individuals. The sequences were aligned and genetic diversity, structure, as well as demographic analyses were performed. Low genetic diversity (π = 0.0007–0.0038 in coding regions and π = 0.0127 in non-coding region; Hd = 0.166–0.569 in coding regions and Hd = 0.584 in non-coding region) and sub-structuring (H= 2–3 in coding regions andH= 4 in non-coding region) was observed with two divergent haplogroups (haplotype pairwise distance = 3–5 in coding region and 6–10 in non-coding region) being identified. This suggests the population may have experienced fixation of mitochondrial haplotypes due to limited female immigration, which is consistent with philopatric species, that alternative haplotypes are not native to this population, and that there may be male mobility from adjacent populations. This study provides the first detailed insights into the mitochondrial genetic diversity of a continental African strepsirrhine primate and demonstrates the utility of mitochondrial DNA in intraspecific genetic population analyses of these primates.