Genetic and functional complementation of the HSV1 UL27 gene and gB glycoprotein by simian alpha-herpesvirus homologs.
Genetic and functional complementation of the HSV1 UL27 gene and gB glycoprotein by simian alpha-herpesvirus homologs.
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猿猴 α-疱疹病毒同源物对 HSV1 UL27 基因和 gB 糖蛋白进行遗传和功能互补。
DOI：
10.1007/s007050050114
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发表时间：
1997
影响因子：
2.7
通讯作者：
Cantin,EM
中科院分区：
文献类型：
--
作者：
Eberle,R;Tanamachi,B;Black,D;Blewett,EL;Ali,M;Openshaw,H;Cantin,EM
将HSV1糖蛋白gB−株的DNA与含有UL26、UL27 （gB糖蛋白）和UL28基因同源物的克隆α-疱疹病毒片段共转染，制备了具有复制能力的重组病毒。对HSVI/SAff8重组体（HSV1/SgB）的遗传分析表明，在HSV1基因组中存在由整个UL27 （gB）基因和部分UL28和UL26 orf组成的SAff8 DNA。结果表明，该重组蛋白表达了SAff8 gB和p40蛋白（分别为UL27和UL26.5基因产物）；所有其他蛋白与HSV1的蛋白难以区分。在gb特异性病毒中和和细胞表面抗体结合试验中，重组蛋白表现得与SAff8相似，而斑块形态和复制动力学与HSV1非常相似。尽管具有压倒性的HSV1基因构成，重组体在小鼠中表现出与亲本HSV1非常不同的致病表型。虽然HSV1在眼部感染的小鼠中产生角膜疾病并容易扩散到神经系统，但HSV1/SgB在这两方面都明显受损。这些结果证明了猴尾猿病毒gB糖蛋白和基因（包括转录调控元件）在HSV1中的功能等效性，HSV1/SAff8嵌合UL28和UL26基因/蛋白的功能性质，以及UL28、gB和/或p40蛋白可能影响HSV1的致病性。
Utilizing co-transfection of DNA from glycoprotein gB−strain of HSV1 and cloned fragments of several simian α-herpesviruses containing the UL26, UL27 (gB glycoprotein), and UL28 gene homologs, replication-compe-tent recombinant viruses were produced. Genetic analysis of one HSVI/SAff8 recombinant (HSV1/SgB) demonstrated the presence of SAff8 DNA comprising the entire UL27 (gB) gene and parts of the UL28 and UL26 ORFs in an otherwise HSV1 genome. The recombinant was shown to express the SAff8 gB and p40 proteins (UL27 & UL26.5 gene products, respectively); all other proteins were indistinguishable from those of HSV1. The recombinant behaved like SAff8 in gB-specific virus neutralization and cell surface antibody binding assays, while plaque morphology and replication kinetics were very similar to HSV1. Despite its overwhelming HSV1 genetic constitution, the recombinant displayed a pathogenic phenotype in mice very different from the parental HSV1. While HSV1 produced corneal disease in ocularly infected mice and readily spread to the nervous system, HSV1/SgB was markedly impaired in both respects. These results demonstrate the functional equivalency of the cercopithecine monkey virus gB glycoproteins and genes (including transcriptional regulatory elements) in HSV1, the funtional nature of HSV1/SAff8 chimeric UL28 and UL26 genes/proteins, and that UL28, gB and/or p40 proteins may effect the pathogenicity of HSV1.