A modified population analysis profile (PAP) method to detect hetero-resistance to vancomycin in Staphylococcus aureus in a UK hospital
A modified population analysis profile (PAP) method to detect hetero-resistance to vancomycin in Staphylococcus aureus in a UK hospital
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DOI：
10.1093/jac/47.4.399
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发表时间：
2001-04-01
影响因子：
5.2
通讯作者：
MacGowan, AP
中科院分区：
文献类型：
--
作者：
Wootton, M;Howe, RA;MacGowan, AP
对1983 ~ 1999年间分离的100株耐甲氧西林金黄色葡萄球菌（MRSA）与耐万古霉素异源金黄色葡萄球菌（hVRSA） Mu 3和耐万古霉素金黄色葡萄球菌（VRSA） Mu 50进行了万古霉素耐药性试验。这是通过Hiramatsu，梯度板，琼脂掺入，标准测试，宏观稀释测试和改良群体分析所描述的筛选方法来实现的。采用Hiramatsu的筛选方法，100个MRSA中有5%被鉴定为VRSA， 5%被鉴定为hVRSA，梯度板鉴定为7% hVRSA，标准试验和宏稀释试验未检测到hVRSA。通过琼脂掺入和标准测试方法，mu3似乎对万古霉素敏感，但通过大量稀释测试，mu3被归类为hVRSA。改良群体分析可靠地检测到Mu 3的万古霉素异源耐药，并且在100份MRSA样本中未发现hvrsa。
One hundred methicillin-resistant Staphylococcus aureus (MRSA) strains, isolated between 1983 and 1999, were tested alongside the vancomycin hetero-resistant S. aureus (hVRSA) strain Mu 3, and vancomycin-resistant S. aureus (VRSA) strain Mu 50, for their resistance to vancomycin. This was achieved using the screening method described by Hiramatsu, gradient plates, agar incorporation, standard Etest, macrodilution Etest and a modified population analysis. Using Hiramatsu's screening method, 5% of the 100 MRSA were identified as VRSA and 5% identified as hVRSA, the gradient plates identified 7% hVRSA, and the standard and macrodilution Etests identified no hVRSA. Mu 3 appeared to be vancomycin-susceptible using both the agar incorporation and standard Etest methods, but was classified as hVRSA using the macrodilution Etest. The modified population analysis reliably detected vancomycin hetero-resistance in Mu 3 and identified no hVRSAs within the 100 MRSA sample.