Plasma Nucleosome Levels in Node-Negative Breast Cancer Patients.
Plasma Nucleosome Levels in Node-Negative Breast Cancer Patients.
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DOI：
10.1007/bf02966454
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发表时间：
1999-10-25
期刊：
影响因子：
--
通讯作者：
Toi
中科院分区：
文献类型：
--
作者：
Kuroi;Tanaka;Toi
背景：核小体是染色质中有组织DNA的主要重复单元，细胞死亡可导致血浆中循环核小体（PNLs）水平升高，如炎症、肺栓塞、自身免疫性疾病和癌症。材料和方法：我们调查了96例0-III期乳腺癌患者的PNLs（淋巴结阴性，n= 57；淋巴结阳性，n=39），以及111名无任何疾病证据的女性作为健康对照。使用细胞死亡检测elisa +试剂盒（Boehringer Mannheim, Japan）检测pnl。结果：正常对照组的PNLs为0.010 +/- 0.012单位（平均+/- SD），而淋巴结阴性乳腺癌患者的PNLs（0.153 +/- 0.242）和淋巴结阳性乳腺癌患者的PNLs (0.116 +/- 0.172) （p 0.10）和低(
BACKGROUND: A nucleosome is a primary repeating unit of organized DNA in chromatin, and cell death may lead to increased levels of circulating nucleosomes inplasma (PNLs) in various circumstances such as inflammation, pulmonary embolism, autoimmune disease and cancer. MATERIALS AND METHODS: We investigated PNLs in 96 patients with stage 0-III breast cancer (node-negative, n =57; node-positive, n=39), and in 111 women without any evidence of disease as healthy controls. PNLs were detected using the Cell Death Detection ELISAplus kit (Boehringer Mannheim, Japan). RESULTS: The PNLs in normal controls were 0.010 +/- 0.012 units (mean +/- SD), while PNLs were significantly higher in both node-negative breast cancer (0.153 +/- 0.242) and node-positive breast cancer patients (0.116 +/- 0.172) (p 0.10) and low(