Cancer cell specific fluorescent methionine protected gold nanoclusters for in-vitro cell imaging studies
Cancer cell specific fluorescent methionine protected gold nanoclusters for in-vitro cell imaging studies
复制标题
用于体外细胞成像研究的癌细胞特异性荧光蛋氨酸保护金纳米簇
DOI：
10.1016/j.talanta.2018.05.079
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发表时间：
2018
期刊：
影响因子：
6.1
通讯作者：
Zhu Manzhou
中科院分区：
文献类型：
--
作者：
Pan Yiting;Li Qinzhen;Zhou Qin;Zhang Wan;Yue Ping;Xu Changzhi;Qin Ximing;Yu Haizhu;Zhu Manzhou
荧光纳米团簇具有良好的光稳定性和生物相容性，近年来成为一种极具吸引力的生物传感和成像材料，特别是在癌症的早期诊断方面。但由于其特异性不高，合成复杂，限制了其临床应用。在这项研究中，新的甲硫氨酸包裹的金纳米团簇（Met-AuNCs）已通过一个容易实现的一锅合成方法制备。所制备的Met-AuNCs具有很高的成像特异性：与Met-AuNCs孵育1 h后，癌细胞（包括A549、Hela、MCF-7、HepG 2）发出荧光，而正常细胞（WI-38和CHO）不发出荧光。根据一系列的对照实验，提出了高成像选择性的原因是源于特异性识别癌细胞中过表达的L-型氨基酸转运蛋白。
Benefiting from the excellent photostability and biocompatibility, fluorescent nanoclusters have recently emerged as a highly attractive bio-sensing and imaging material, especially in early diagnosis of cancer. However, their clinic applications were limited by the unsatisfactory specificity and the complex synthesis. In this study, novel methionine coated gold nanoclusters (Met-AuNCs) have been prepared via an easily-achievable one-pot synthetic method. The prepared Met-AuNCs showed high imaging-specificity: after incubating with Met-AuNCs for 1 h, cancer cells (including A549, Hela, MCF-7, HepG2) were fluorescent, while the normal cells (WI-38 and CHO) showed no fluorescence. According to a series of controlled experiments, the reason for the high imaging-selectivity was proposed to originate from the specific recognition of L-type amino acid transporter overexpressed in cancer cells.