Paeoniflorin attenuates allergic inflammation in asthmatic mice
Paeoniflorin attenuates allergic inflammation in asthmatic mice
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芍药苷减轻哮喘小鼠的过敏性炎症
DOI：
10.1016/j.intimp.2014.11.016
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发表时间：
2015-01-01
影响因子：
5.6
通讯作者：
Dong, Jingcheng
中科院分区：
文献类型：
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作者：
Sun, Jing;Wu, Jinfeng;Dong, Jingcheng
芍药苷（PF）是中国牡丹的主要活性成分之一，具有抗炎和免疫调节作用。然而，目前尚不清楚 PF 治疗是否可以抑制哮喘的过敏性炎症。在这项研究中，我们评估了 PF 对哮喘小鼠肺功能和气道炎症的影响。采用BALB/c小鼠建立过敏性哮喘模型。对小鼠进行致敏并用卵清蛋白进行攻击。通过直接气道阻力分析检测气道高反应性。检查肺组织的炎症细胞浸润。分别采用ELISA和实时PCR检测支气管肺泡灌洗液（BALF）中的IL-5、IL-13、IL-17和eotaxin及其在肺组织中的mRNA表达量。通过ELISA测定血清中总IgE水平。 Western blot检测p-ERK和p-JNK蛋白表达。我们的数据显示，口服 PF 显着降低气道对雾化乙酰甲胆碱的高反应性，降低 BALF 中的 IL-5、IL-13、IL-17 和嗜酸细胞活化趋化因子水平，并降低血清中的 IgE 水平。组织学研究表明，PF 给药显着减少了炎症浸润。同样，PF 治疗显着抑制肺组织中 IL-5、IL-13、IL-17 和嗜酸细胞趋化因子 mRNA 的表达。口服PF后p-ERK和p-JNK蛋白表达水平显着降低。总之，PF 通过降低 IL-5、IL-13、IL-17 和嗜酸细胞趋化因子的表达，在 OVA 诱导的哮喘模型中发挥抗炎作用。这些作用至少部分是通过抑制 MAPK 途径的激活来介导的。 (C) 2014 Elsevier B.V. 保留所有权利。
Paeoniflorin (PF), one of the major active ingredients of Chinese peony, has demonstrated anti-inflammatory and immunoregulatory effects. However, it has remained unclear whether PF treatment can inhibit allergic inflammation in asthma. In this study, we evaluated the effects of PF on pulmonary function and airway inflammation in asthmatic mice. The allergic asthma models were established in BALB/c mice. The mice were sensitized and challenged with ovalbumin. Airway hyperresponsiveness was detected by direct airway resistance analysis. Lung tissues were examined for inflammatory cell infiltration. IL-5, IL-13, IL-17, and eotaxin in bronchoalveolar lavage fluid (BALF) and their mRNA expression in lung tissue were examined by ELISA and realtime PCR, respectively. The total IgE level in serum was measured by ELISA. The protein expression of p-ERK and p-JNK was detected by western blot. Our data showed that PF oral administration significantly reduced airway hyperresponsiveness to aerosolized methacholine and decreased IL-5, IL-13, IL-17 and eotaxin levels in the BALF, and decreased IgE level in the serum. Histological studies showed that PF administration markedly decreased inflammatory infiltration. Similarly, treatment with PF significantly inhibited IL-5, IL-13, IL-17 and eotaxin mRNA expression in lung tissues. The protein expression levels of p-ERK and p-JNK were substantially decreased after oral administration of PF. In summary, PF displayed anti-inflammatory effects in the OVA-induced asthmatic model by decreasing the expression of IL-5, IL-13, IL-17 and eotaxin. These effects were mediated at least partially by inhibiting the activation of MAPK pathway. (C) 2014 Elsevier B.V. All rights reserved.