Activation of human natural killer cells by herpes simplex virus type 1-infected cells.
Activation of human natural killer cells by herpes simplex virus type 1-infected cells.
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1 型单纯疱疹病毒感染细胞激活人类自然杀伤细胞。
DOI：
10.1159/000149999
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发表时间：
1987
期刊：
影响因子：
4.6
通讯作者：
Glorioso,JC
中科院分区：
文献类型：
--
作者：
Bishop,GA;McCurry,L;Schwartz,SA;Glorioso,JC
在18小时51 Cr释放试验中，人自然杀伤（NK）细胞对单纯疱疹病毒1型（HSV-1）感染的WISH细胞的细胞溶解性比未感染的细胞大得多。涉及高冷靶与放射性标记的靶细胞比率的冷靶竞争实验证明，感染的细胞特异性地竞争针对感染的细胞靶的裂解活性，因此，感染的细胞并不固有地比未感染的细胞对裂解更敏感。与这些发现相反，涉及低比率的冷HSV-1感染的靶细胞与标记的感染的靶细胞的消耗实验导致对未感染的细胞靶的裂解活性增加。这一发现表明，对WISH细胞表面决定簇具有特异性的NK效应子在耗竭孵育期间变得高度活化。在NK细胞毒性测定中加入干扰素α，特别是白细胞介素2，以剂量依赖性方式增强了对感染和未感染靶细胞的NK细胞溶解活性。白细胞介素1没有这种效果。然而，暴露于低剂量的感染细胞冷靶后NK细胞的裂解活性增强不能仅基于测试的三种淋巴因子的释放来解释，因为白细胞介素1是无效的，在来自竞争实验的培养上清液中未检测到白细胞介素2，并且NK细胞优先裂解HSV-1感染的靶细胞，而不依赖于干扰素的增强作用。总之，结果表明NK细胞识别并结合特定的靶细胞表面结构，这反过来可能增强其裂解活性。
Human natural killer (NK) cells were shown to be much more cytolytic for WISH cells infected with herpes simplex virus type 1 (HSV-1) than for uninfected cells during 18-hour51Cr release assays. Cold-target competition experiments involving high cold target to radiolabeled target cell ratios demonstrated that infected cells specifically competed for lytic activity against infected cell targets, and, therefore, the infected cells were not inherently more sensitive to lysis than uninfected cells. In contrast to these findings, depletion experiments involving low ratios of cold HSV-1-infected targets to labeled infected target cells resulted in increased lytic activity against uninfected cell targets. This finding suggested that NK effectors with specificity for WISH cell surface determinants had become highly activated during the depletion incubation. The addition of interferon alpha and particularly interleukin 2 to NK cytotoxicity assays enhanced NK cytolytic activity against both infected and uninfected target cells in a dose-dependent manner. Interleukin 1 did not give this effect. However, the enhanced lytic activity of NK cells following exposure to low doses of infected cell cold targets cannot be explained solely on the basis of release of the three lymphokines tested, since interleukin 1 was not effective, interleukin 2 was not detected in culture supernatants derived from the competition experiments, and NK cells preferentially lyse HSV-1-infected target cells independent of the enhancing effects of interferon. Together, the results indicated that NK cells recognize and bind to specific target cell surface structures which may, in turn, enhance their lytic activity.