Prenatal alcohol exposure decreases the number of nitric oxide synthase positive neurons in rat superior colliculus and periaqueductal gray.
Prenatal alcohol exposure decreases the number of nitric oxide synthase positive neurons in rat superior colliculus and periaqueductal gray.
复制标题
产前酒精暴露会减少大鼠上丘和导水管周围灰质中一氧化氮合酶阳性神经元的数量。
DOI：
10.1016/s0741-8329(00)00108-7
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Wall,KA
中科院分区：
文献类型：
--
作者：
Phillips,DE;Cummings,JD;Wall,KA
由于一氧化氮（NO）参与轴突突起和突触的发育和完善，因此了解发育性酒精暴露是否影响产生NO的神经元是很有意义的。怀孕大鼠在整个妊娠期间喂食人工液体饲料作为唯一的液体或热量来源。试验坝饲粮中添加乙醇（6.7% v/v），对照坝配对饲粮中添加等热量麦芽糖-糊精替代乙醇。已知这种乙醇饮食方案产生的血液酒精浓度峰值约为140毫克%。在出生后第15天（P15）和35天（P35），分别在上丘脑（SC）浅灰层（SGS）和导水管周围灰质（dlPAG）背外侧柱的横截面上计数组织化学染色的一氧化氮合酶（NOS）细胞。与对照组织相比，酒精引起的影响如下：在SC中，P15时NOS+神经元的面密度下降24%，但P35时平均下降幅度相似，无统计学意义（P=0.10）；体大小在P15或P35均未受影响。dlPAG的面密度和NOS+神经元总数在P15时未受影响，但在P35时下降（分别下降33%和37%）；体大小在P15或P35均未受影响。P15时SC中NOS+神经元的减少可能会对神经元连接的细化产生负面影响，而P35时dlPAG中NOS+神经元的减少可能代表更持久的影响，可能会改变该核的功能。
Because nitric oxide (NO) is involved in the development and refinement of axonal projections and synapses, it is of interest to know if developmental alcohol exposures affect NO producing neurons. Pregnant rats were fed artificial liquid diet throughout gestation as the only fluid or caloric source. The diet for experimental dams contained ethanol (6.7% v/v) while the pair-fed diet for control dams contained isocaloric maltose–dextrin instead of ethanol. This ethanol diet regime is known to produce peak blood alcohol concentrations of approximately 140 mg%. Cells stained histochemically for nitric oxide synthase (NOS) were counted at postnatal day 15 (P15) and 35 (P35) in cross-sections of the stratum griseum superficiale (SGS) of the superior colliculus (SC) and in the dorsolateral column of the periaqueductal gray (dlPAG). Compared to control tissues, alcohol caused the following effects: In the SC, the areal density of NOS+ neurons was decreased 24% at P15 but a similar decrease in means at P35 was not statistically significant (P=0.10); soma size was unaffected at either P15 or P35. In the dlPAG, both the areal density and the total number of NOS+ neurons per section were unaffected at P15 but were decreased at P35 (33% and 37% decreases); soma size was unaffected at either P15 or P35. The decrease in NOS+ neurons in the SC at P15 could be expected to have a negative impact on the refinement of neuronal connections while the decreases in NOS+ neurons in the dlPAG at P35 likely represent more permanent effects that could alter the function of that nucleus.