Abelson virus-infected cells can exhibit restricted in vitro growth and low oncogenic potential.
Abelson virus-infected cells can exhibit restricted in vitro growth and low oncogenic potential.
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阿贝尔森病毒感染的细胞可以表现出体外生长受限和低致癌潜力。
DOI：
10.1128/jvi.40.2.577-584.1981
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发表时间：
1981
影响因子：
5.4
通讯作者：
Witte,ON
中科院分区：
文献类型：
--
作者：
Whitlock,CA;Witte,ON
我们设计了一种培养感染阿贝尔森鼠白血病病毒的骨髓细胞的方法，该方法允许在感染后早期检查靶细胞的生长。该培养系统有利于原代培养物中贴壁细胞混合群的快速生长，从而提高了靶细胞生长的效率。非贴壁的 Abelson 病毒感染细胞群表达 Abelson 病毒转化细胞特有的前 B 细胞分化标记物（免疫球蛋白 M 的 mu 重链和末端脱氧核苷酸转移酶）。感染后早期，这些细胞群表现出受限的体外和体内生长特性，这与已建立的阿贝尔森病毒转化细胞系 2M3 不同。这些包括生长和活力对贴壁细胞层的明显依赖性、琼脂集落形成效率较低以及同系动物中肿瘤产生能力较低。通过使用在没有病毒感染的情况下建立的长期贴壁细胞培养物的条件培养基，可以在没有贴壁细胞层的情况下维持早期群体的生长。群体传代几周后，体外生长特性逐渐转向 2M3 细胞系。十二周龄的群体独立于贴壁细胞层生长，并且显示出琼脂集落形成效率的提高。这些数据表明，许多淋巴靶细胞在感染阿贝尔森病毒时表现出中间转化的表型。这些细胞在培养物中的生长是通过病毒转化基因的细胞内表达与可由贴壁骨髓细胞培养物提供的外源生长促进活性之间的协同相互作用介导的。
We have designed a method for growing bone marrow cells infected with Abelson murine leukemia virus which permits examination of target cell growth early after infection. This culture system increases the efficiency of target cell growth by favoring rapid growth of a mixed population of adherent cells in the primary culture. The nonadherent Abelson virus-infected cell populations expressed pre-B-cell differentiation markers characteristic of Abelson virus-transformed cells (mu-heavy chains of immunoglobulin M and terminal deoxynucleotidyltransferase). Early after infection, these cell populations exhibited restricted in vitro and in vivo growth properties which differed from those of an established Abelson virus-transformed cell line, 2M3. These included a marked dependency upon the adherent cell layer for growth and viability, a lower efficiency of agar colony formation, and a lower capacity for tumor production in syngeneic animals. Growth of the early populations could be maintained in the absence of the adherent cell layer by using conditioned medium from long-term adherent cell cultures established in the absence of viral infection. After passage of the populations for several weeks, the in vitro growth properties gradually shifted toward that of the 2M3 cell line. Twelve-week-old populations grew independently of the adherent cell layer and showed an increased efficiency of agar colony formation. These data indicate that many lymphoid target cells exhibit an intermediate transformed phenotype when infected with Abelson virus. Growth of these cells in culture is mediated via a synergistic interaction between intracellular expression of the viral transforming gene and an exogenous growth-promoting activity which can be provided by cultures of adherent bone marrow cells.