Degradation of electrospun PLGA-chitosan/PVA membranes and their cytocompatibility in vitro
Degradation of electrospun PLGA-chitosan/PVA membranes and their cytocompatibility in vitro
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电纺PLGA-壳聚糖/PVA膜的降解及其体外细胞相容性
DOI：
10.1163/156856207779146105
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发表时间：
2007-01-01
影响因子：
3.6
通讯作者：
Yao, Kangde
中科院分区：
文献类型：
--
作者：
Duan, Bin;Wu, Lili;Yao, Kangde
通过从两个不同注射器同时静电纺丝 PLGA 和壳聚糖/PVA 制备纳米纤维复合聚丙交酯乙交酯 (PLGA) 和壳聚糖/聚乙烯醇 (PVA) 膜。在 37 摄氏度下，在磷酸盐缓冲盐水（PBS，pH 7.4）中检查 PLGA 和交联复合膜的体外降解长达 10 周。测量 PBS 的 pH 值、PLGA 的重均分子量、纤维形态和机械性能（包括拉伸强度、杨氏模量和断裂伸长率）作为降解时间的函数。进一步研究了纤维复合膜作为人胚胎皮肤成纤维细胞（hESF）培养的有前景的支架。赖特-吉姆萨染色后，使用扫描电子显微镜和倒置相差显微镜观察接种在每个电纺膜上的 hESF 的细胞粘附和形态。壳聚糖/PVA组分的引入改变了亲水/疏水平衡，从而影响了复合膜在降解过程中的降解行为和机械性能。细胞不仅可以在复合膜上良好地附着和生长，而且还能够迁移和渗透膜。因此，结果表明复合膜可以很好地模拟天然细胞外基质的结构，并具有作为三维组织工程支架的应用潜力。
Nanofibrious composite poly(lactide-co-glycolide) (PLGA) and chitosan/poly(vinyl alcohol) (PVA) membranes were prepared by simultaneously electrospinning PLGA and chitosan/PVA from two different syringes. The in vitro degradation of PLGA and cross-linked composite membranes was examined for up to 10 weeks in phosphate-buffered saline (PBS, pH 7.4) at 37 degrees C. The pH of PBS, the weight average molecular weight of PLGA, fiber morphology and mechanical properties, including tensile strength, Young's modulus and elongation-at-break, were measured as a function of degradation time. The fibrous composite membranes were further investigated as a promising scaffold for human embryo skin fibroblasts (hESFs) culture. The cell adhesion and morphology of hESFs seeded on each electrospun membrane was observed using scanning electron microscope and inverted phase contrast microscopy after Wright-Giemsa staining. The introduction of chitosan/PVA component changed the hydrophilic/hydrophobic balance and, thus, influenced degradation behavior and mechanical properties of the composite membranes during degradation. The cells could not only favorably attach and grow well on the composite membranes, but were also able to migrate and infiltrate the membranes. Therefore, the results suggest that the composite membranes can positively mimic the structure of natural extracellular matrices and have the potential for application as three-dimensional tissue-engineering scaffolds.