Improved tissue adhesion property of a hydrophobically modified Alaska pollock derived gelatin sheet by UV treatment
Improved tissue adhesion property of a hydrophobically modified Alaska pollock derived gelatin sheet by UV treatment
复制标题
通过紫外线处理改善疏水改性阿拉斯加鳕鱼衍生明胶片的组织粘附性能
DOI：
10.1016/j.ijbiomac.2021.01.085
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发表时间：
2021
影响因子：
8.2
通讯作者：
Taguchi Tetsushi
中科院分区：
文献类型：
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作者：
Ichimaru Hiroaki;Taguchi Tetsushi
已经开发了组织粘合剂用于密封手术中受损的组织。其中，片状粘合剂在潮湿条件下需要相对长的时间来粘附到生物组织。为了解决这一临床问题，我们利用静电纺丝制备了基于癸基（C10）改性的阿拉斯加鳕鱼衍生明胶（C10-ApGltn）的组织粘附纤维片（AdFS）。对AdFS进行紫外线照射，通过引入亲水性官能团来增加AdFS与湿生物组织之间的亲和力。经紫外线照射的AdFS（UV-C10-AdFS）在潮湿条件下在2 min内与猪胸膜强烈粘附，并且与原始ApGltn（Org-ApGltn）片材相比显示出更高的破裂强度。苏木精-伊红染色切片显示，即使在破裂强度测量后，致密的UV-C10-AdFS层仍保留在猪胸膜表面上。此外，UV-C10-AdFS具有良好的细胞相容性，并有效地支持L929细胞的生长。UV-C10-AdFS是一种很有前途的湿生物组织封接材料。
Tissue adhesives have been developed for sealing tissue damaged in surgery. Among these, sheet-type adhesives require a relatively long time to adhere to biological tissue under wet conditions. To address this clinical problem, we fabricated a tissue-adhesive fiber sheet (AdFS) based on decanyl group (C10) modified Alaska pollock-derived gelatin (C10-ApGltn) using electrospinning. Ultraviolet (UV) irradiation of the AdFS was performed to increase the affinity between the AdFS and wet biological tissue by introducing hydrophilic functional groups. The UV irradiated AdFS (UV-C10-AdFS) strongly adhered to porcine pleura within 2 min under wet conditions and showed higher burst strength compared with the original ApGltn (Org-ApGltn) sheet. Hematoxylin-eosin stained sections revealed that a dense UV-C10-AdFS layer remained on the surface of the porcine pleura even after burst strength measurement. Moreover, UV-C10-AdFS has excellent cytocompatibility and efficiently supports the growth of L929 cells. UV-C10-AdFS is a promising adhesive material for sealing wet biological tissue.